红藻氨酸诱导癫痫发作小鼠脑组织中 NO_2~- 与硫代巴比妥酸反应物浓度测定

了解活性介质一氧化氮（ＮＯ）与癫痫发作的关系。方法用比色法检测红藻氨酸（ＫＡ）诱导ＢＡＬＢ／ｃ小鼠癫痫发作后，不同时点脑匀浆上清液中亚硝酸根（ＮＯ２－）与硫代巴比妥酸（ＴＢＡ）反应物的含量。结果ＮＯ２－浓度与ＴＢＡ反应物含量的多少与ＫＡ诱导的癫痫发作时程有关。在发作初期，ＮＯ２－随发作持续而增多，但发作后期又迅速减少。ＮＯ２－浓度的升高与减低受Ｌ－精氨酸（Ｌ－Ａｒｇ）及其硝基衍生物ＮＧ位硝基左型精氨酸（Ｌ－ＮＮＡ）的影响。同时对ＮＯ２－检测标本做ＴＢＡ反应物检测，发现ＮＯ２－的减少与ＴＢＡ反应物增多相关，在癫痫发作初期ＴＢＡ反应物含量较低，但随着发作时间的延长明显增多，ＴＢＡ含量的变化同样可受Ｌ－Ａｒｇ与Ｌ－ＮＮＡ的影响。结论Ｌ－Ａｒｇ－ＮＯ途径可能参与了癫痫发作的起动、传播和继发性脑损害的全过程，在发作后期，过量生成的ＮＯ可能有神经兴奋毒性作用。

Colorimetric determination of concentration of nitrite and thiobarbituric acid reactive substances in mouse brain during kainic acid induced seizures

Objective The study was to explore the implication of nitric oxide (NO) pathway in epilepsy.Methods The concentrations of the nitrite (NO 2 -) and thiobarbituric acid reactive substances (TBARSs) in the brain homogenate of BALB/c mice were determined with the colorimetric methods. Results Both the production of NO 2 - and TBARSs were related to the different stages of epileptic seizures induced by kainic acid (KA). NO 2 - was highly increased but TBARSs were kept at low levels at the early seizure stage ( P <0.01). Along with the seizure lasting, NO 2 - decreased but TBARSs increased rapidly ( P <0.01). The concentration variations of both NO 2 - and TBARSs were influenced by L arginine ( P <0.01) and N G nitro L arginine ( P <0.05). Conclusion The L Arg NO pathway was definitely involved in the KA seizure. During the epileptic seizures, NO might firstly act as a homeostasis agent and thereafter it might insult the brain.