CK19/20检测对结直肠癌微转移的诊断意义

目的 探讨 CK19/20mRNART-PCR检测对结直肠癌微转移的诊断意义。方法 采用RT-PCR逆转录多聚酶链反应）方法检测42例结、直肠癌患者及对照组6例非恶性病变腹部手术患者骨髓血、门静脉血和外周血CK19mHNA及 CK20 mRNA的表达。结果 对照组6例患者无 CK19及 CK20阳性表达,CK19及CK20在42例结、直肠癌患者骨髓血、门静脉血及外周血中阳性表达分别为24/42、21/42、11/42及27/42、18/42、11/42,两间无显著差异（P>0.05）,骨髓和门静脉中表达比外周血高（P<0.01）,Dukes　C 期患者表达高于Dukes A、B期（P<0.05）。结论 CK19、CK20的RT-PCR检测是较敏感和特异的结、直肠癌微小转移指标,阳性表达患者有较高发生远处转移的危险。

Clinical significance of CK19/20 in colorectal cancer patients

Objective To value RT- PCR for CK19/20 in diagnosis of micrometastasis in colorectal cancer and its clinical significance. Methods CK19/20 mRNA by was investigated RT- PCR in bone marrow, portal vein and peripheral blood in 42 patients and 6 volunteers. Results CK19/20 was not detected in 6 volunteers. The positive rate of CK19 and CK20 in bone marrow, portal vein, peripheral blood were 57.1%(24/42), 50% (21/42), 26.2%(ll/42) and64.3%(27/42), 42.9%(18/42), 26.2%(ll/42). The positive rates in dukes C stage were significantly higher than Dukes B and Dukes A stages. Conclusions RT- PCR for CK19/20 is a sensitive and specific method to diagnose the micrometastasis of colorectal cancer. The patients whose CK19/20 are positively expressed are in high risk of metastasis.