完整肽聚糖对裸鼠腹腔巨噬细胞分泌和杀瘤功能的影响

目的：探索靶向性非病毒载体系统在人脑腕质瘤基因治疗中的应用。方法：组了ＥＧＦ－Ｒ介导的ＧＥ７基因转移系统,分别与β－ａｌ报道基因和Ｐ２１基因构成载体复合物,体外转染Ｕ２５１细胞,通过Ｘ－ｇａｌ染色、Ｗｅｓｔｅｒｎ ｂｌｏｔ分析,原位木端标记Ｄ梯带检测等,观察了外源基因的导入及对肿瘤细胞的抑制作用。结果：Ｘｇａｌ染色表明,外源基因的导入率可达８０％。转染Ｐ２１基因后,细胞生长受到明显抑制第７天生长抑

The Effect of Whole Peptidoglycan of Bifidobacterium on Cytokines and Cytotoxity Activity Derived from Peritoneal Macrophages of Nude Mice

Objective To investigate the effects ofnovel targeted non-viral vector in gene therapy of human glioma. Methods The EGF-Rtargeting gene delivery system GE7 was constructed. Human glioma cell line U251 wastransfected in vitro with β-gal as reportor gene and p21 as therapeutic gene using this gene deliverysystem. By means of the assay of β-galactosidase staining, Western blotting, in situ endlabeling apoptosis cells and DNA ladder, the transferring of exogenous genes and theapoptosis of the tumor cells were examined. Results It was showed that gene transferefficiency is over 80%. When transfected with p21 gene, the growth of cells was inhibitedsignificantly, and the apoptosis was detected in the transfected cell by the methods of insitu end labeling and DNA ladder. Conclusion The GE7 gene delivery system has the abilityto transfer exogenous gene to tumor cells and the expression of the therapeutic gene caninhibit the growth of the cells.