运动训练对脑梗死大鼠学习记忆能力的影响

背景学习和记忆的神经基础是中枢神经系统具有高度的可塑性,在中枢神经系统功能重组的过程中需要特定的康复训练.目的观察运动训练对大脑中动脉梗死模型大鼠在实验中增强分辨学习能力和一次性被动回避反应的记忆保持能力的作用.设计随机对照实验.单位泸州医学院附属医院康复医学科.材料实验于2002-07/2003-12在泸州医学院动物实验室完成.实验动物为8周龄Wistar雄性大鼠24只,随机分为脑梗死自由活动组(模型组)、脑梗死运动训练组(康复组)和正常组,每组8只.方法①模型制备康复组和模型组大鼠建立右侧大脑中动脉梗死模型,正常组不做处理.②运动训练4 d后对康复组大鼠进行4周的滚笼训练器、网屏训练器、平衡训练器训练,模型组和正常组不进行特殊的运动训练.③学习记忆测试各组大鼠于术后第35天进行学习记忆行为学测试.Y型迷宫实验主要观察大鼠达到9/10正确反应(跑到暗臂)所需的训练次数;多功能条件反射箱实验主要观察大鼠在跳板上停留的时间(步入潜伏期).主要观察指标①各组大鼠达到常握迷宫结构标准所需的训练次数.②各组大鼠步入潜伏期.结果24只大鼠均进入结果分析.①Y迷宫实验检测结果康复组和正常组达到掌握迷宫结构标准所需的训练次数分别是(68.02±11.67)次和(57.62±10.31)次,而模型组则需要(107.07±16.32)次,模型组明显多于康复组和对照组(t=2.05,P＜0.05).②多功能条件反射箱实验结果对大鼠一次性被动回避反应的记忆保持能力测试中显示,康复组和正常组步入潜伏期中位数分别是286.7 s和298.4 s,模型组为126.7 s,模型组明显短于康复组和正常组(T=48,P＜0.05).结论运动训练可以促进脑梗死大鼠学习记忆能力的恢复.

Effect of physical training on learning and memory ability in rats with cerebral infarction

BACKGROUND: Neural basis of learning and memory is the great plasticity of the central nervous system. Therefore, special rehabilitative training is needed in the process of functional reconstruction of the central nervous system.OBJECTIVE: To observe the effects of physical training in enhancing differential learning ability and memory-maintaining ability of one-off passive avoidance reaction in rats with middle cerebral artery infarction.DESIGN: Randomized controlled trial.SETTING: Department of Rehabilitation Medicine, Affiliated Hospital of Luzhou Medical College.MATERIALS: This experiment was conducted in the Animal Laboratory of Luzhou Medical College between July 2002 and December 2003. Totally 24 male Wistar rats aged 8 weeks were recruited and randomized into cerebral infarction and freely moving group (model group), cerebral infarction and physical training group (rehabilitation group), and normal control group with 8 rats in each group.artery infarction was established in rats of rehabilitation group and model cal training: Four days later, rats in rehabilitation group were given 4-week training on rolling-cage exerciser, screen exerciser, and balancing exerciser;rats in model group and normal control group were given no special physical en learning and memory praxiology test 35 days after operation. Y-maze test was to observe training times for reaching 9/10 accurate response (to the dark brachium); multi-functional conditioning box was used to observe the time of rats' staying on the diving board (entering latency).maze test: The training times needed by rats in rehabilitation group and normal control group to meet the structural standard of maze was (68.02±11.67) times and (57.62±10.31) times, whereas in model group it was (107.07±16.32)times. The number of training times in model group was obviously much greatly than sults of multi-functional conditioning box: Test of memory-maintaining ability of one-off passive avoidance reaction showed that the median of latency of the rats in rehabilitation group and normal group was 286.7 s and 298.4 s, respectively, and in model group it was 126.7 s, which was obviously shorter than that in rehabilitation group and normal group (T=48, P ＜ 0.05).CONCLUSION: Physical training can promote the recovery of learning and memory ability of rats with cerebral infarction.