丙泊酚对大鼠肺缺血-再灌注损伤的影响

目的 探讨丙泊酚对大鼠肺缺血-再灌注(I-R)损伤的作用及其机制.方法 钳闭大鼠左肺门45 min,再灌注2 h,建立在体肺I-R模型.动物随机分为四组:假手术组、缺血组(夹闭45min,但不再灌注)、I-R组和丙泊酚组(I R并使用丙泊酚麻醉).监测动脉氧分压(PaO2),测定左肺湿干比(W/D)和左肺支气管肺泡灌洗液(BALF)中丙二醛(MDA)、总蛋白及磷脂浓度.用流式细胞仪测定全血中性粒细胞CD18的表达和呼吸爆发.结果 再灌注2 h各组PaO2差异无统计学意义.与假手术组比较,I-R组BALF中的MDA、总蛋白浓度和W/D均升高(P＜0.05),磷脂含量降低(P＜0.01),以上指标丙泊酚组与假手术组比较差异无统计学意义.I-R组中性粒细胞CD18的表达和呼吸爆发均高于丙泊酚组(P＜0.05).结论 丙泊酚对大鼠在体肺I R损伤具有保护作用,其机制可能与其抗氧化作用和抑制中性粒细胞作用有关.

Protective effects of propofol on pulmonary ischemia-reperfusion injury in rats

Objective To investigate the effects of propofol on ischemia-reperfusion(I-R)injury in rat lung and its mechanism.Methods A rat model of pulmonary warm I-R injury was established by occlusion of the left lung hilus for 45 min and then reperfused for 2 h.Animals were divided into four groups randomly:sham operation group(without occlusion),ischemia group(without reperfusion),I-R group,and propofol group(I-R with propofol anesthesia).We measured PaO2,wet to dry(W/D)weight ratio of left lung,the level of MDA,total protein and phospholipid in bronchoaveolar lavage fluid(BALF)of left lung,the CD18 expression and respiratory burst(RB)of neutrophils by flow cytometry.Results There were no significant differences in PaO2 among four groups.The levels of MDA,total protein in BALF and W/D ratio in I-R group were significantly higher than those in propofol group and sham operation group(P<0.05).The level of phospholipid in BALF was lower than that in propofol group and sham operation group(P<0.01).CD18 expression and RB of neutrophils in I-R group were higher than those in propofol group(P<0.05).Conclusion Propofol had a protective effect on pulmonary I-R injury in rats.The mechanism may be related to the antioxidant effect and the suppression of the neutrophils activity.