维生素K3增强三氧化二砷诱导多发性骨髓瘤细胞凋亡的实验研究

目的:探讨维生素K3对三氧化二砷(As2O3)诱导人多发性骨髓瘤(MM)细胞株RPMI8226细胞凋亡的作用及生长抑制。方法:采用四甲基噻唑氮蓝比色法检测As2O3联合维生素K3对人多发性骨髓瘤(MM)细胞株RPMI8226细胞生长抑制作用;流式细胞术检测As2O3联合维生素K3对RPMI8226细胞周期和凋亡率的影响。结果:单用As2O3对RPMI8226细胞的生长有抑制作用,其作用呈时间和剂量依赖性;与单用As2O3相比,As2O3联合维生素K3作用于RPMI8226细胞48h后,细胞凋亡率显著增加(P<0.05),G0/G1期细胞比例升高,S期减低,并有凋亡峰。结论:维生素K3可以增强As2O3对RPMI8226细胞诱导凋亡作用。

VK3 Enhances Apoptosis of Multiple Myeloma RPM I 8226 Cells Induced by Arsenic Trioxide

Objective：To investigate the effect of VK3 on arsenic trioxide induced apoptosis of muhiple myeloma RPMI8226 cells and the molecular mechanisms. Methods：Growth inhibition of RPMI8226 cells exposed to Vitamin K3 Combination with As2 03 was analyzed by MTY assay, Annexin V labeling. Results：As2 03 alone could inhibit the growth and induce apoptosis of RPMI 8226 cells, while As2 03 inhibited the proliferation of RPMI8226 cells in a time and dose - dependent manner. Compared with As2 03 treatment alone, growth inhi- bition was enhanced by combination therapy with As2 03 and VK3 （P 〈 0. 05 ）., Annexin V staining showed the earlier apoptotie cells. Flow cytometry showed that the proportion of G0/GI cells was increased and cell proportion in phase was decreased with appearance of apopto- sis peak. Conclusion：VK3 enhances the apoptosis induced by arsenic trioxide in RPMI8226 cells.