寿胎丸提取液透过妊娠大鼠胎盘屏障的药物成分研究

目的:对不同孕期妊娠大鼠给予寿胎丸提取液(STW),采用HPLC研究STW透过胎盘屏障的药物成分。方法:早、中、晚孕期Wistar大鼠灌胃STW,连续给药5天后,取母体血浆(母血)、脐静脉血及胚胎组织,用HPLC法检测其中所含药物成分。结果:在实验剂量下,各孕期1h母血中均检测到马钱子苷和槲皮苷,12h母血、脐静脉血及胚胎组织中均未检测到待测成分。结论:实验剂量下,STW的药物成分未透过胎盘屏障,提示妊娠期应用寿胎丸不会造成胎儿的宫内暴露。

Study on ingredients of Shou Tai Wan extract through placental barrier of rat in pregnancy

Objective：Use HPLC to study the permeation of ingredients of Shou Tai Wan extract （STW） through placental barriers of Wistar rats at different stages of pregnancy. Methods ： The pregnant rats were administered by gavage STW for 5 days at early, middle and late stages of pregnancy. Hepatic portal vein plasma,umbilical veins plasma （at late stage of pregnancy） and embryonic tissues were obtained 1 h, 12 h after the final administration of STW. The compounds in biological specimens were identified by HPLC. Results：Among the range of experimental doses, loganin and quercitrin were main compounds in 1 h maternal blood but nothing could examined in 12 h maternal blood, umbilical veins plasma and embryonic tissues at different stages of pregnancy. Conclusion： Under the range of experimental doses,the compounds of STW didn＇ t permeate through placental barriers of rats. This result showed that the application of STW is safe to embryo in pregnancy.