pEZR~(Thr567)在肺鳞癌中的表达及临床病理学意义

目的:探讨第567位苏氨酸磷酸化的埃兹蛋白(p EZRThr567)在肺鳞癌、癌旁及正常肺组织中的表达及其临床病理学意义。方法:用免疫组化法检测p EZRThr567在肺癌、癌旁及正常肺组织中的表达,并分析其异常表达与临床病理学特点之间的相关性;用免疫荧光染色法检测p EZRThr567在肺鳞癌细胞EBC-1中的定位情况。结果:p EZRThr567蛋白在肺鳞癌组织中的表达明显高于癌旁组织和正常肺组织(P0.01);p EZRThr567蛋白主要表达于细胞膜,且其表达与肿瘤分化程度、临床分期和有无淋巴结转移密切相关(P0.05)。结论:p EZRThr567蛋白可作为肺鳞癌恶性进展的有效分子指标。

Clinicopathological significance of pEZRThr567 expression in lung squamous carcinoma

AIM: To investigate the clinicopathological significance of the protein expression of phosphorylated ezrin at threonine 567 (pEZR^Thr567) in lung squamous cell carcinoma, adjacent tissues and normal tissues. METHODS: pEZR^Thr567 protein was detected in lung squamous carcinoma, adjacent and normal tissues by the method of immunohistochemistry. The correlation of pEZR^Thr567 expression with clinicopathological parameters of lung squamous carcinomas was also analyzed. The localization of pEZR^Thr567 was detected by immunofluorescence staining in lung squamous cell line EBC-1. RESULTS: The protein expression of pEZR^Thr567 in lung squamous carcinoma was significantly higher than that in the adjacent and normal lung tissues (P<0.01). pEZR^Thr567 mainly localized on the cell membrane, and its over-expression signi-ficantly correlated with the differentiation, clinical stage and lymph node metastasis in lung squamous carcinoma. CONCLUSION: pEZR^Thr567 may be an effective biomarker for prediction of malignant potential and poor prognosis of lung cancer.