中国大陆日本血吸虫品系的研究——Ⅻ.五地隔离群血吸虫DNA杂交

以pSM889为探针,对分布于中国大陆安徽、湖北、广西、云南、四川5地日本血吸虫自然隔离群的雄虫DNA进行了杂交,分析其长度多态性差异。经限制性内切酶EcoRI和BamHI酶切的杂交图谱分析,结果表明其强杂交带虽相同,但在EcoRI酶切的杂交图谱中,分子量分别在4.4-9.6kb和2.3-4.4kb的上述5地隔离群的弱杂交带上却呈现明显的差别。这表明5地自然隔离群日本血吸虫的DNA结构发生了某些变异,此结果进一步为中国大陆境内日本血吸虫存在品系差别提供了分子生物学的依据。

STUDIES ON THE STRAIN DIFFERENCES OF SCHISTOSOMA JAPONICUM IN THE MAINLAND OF CHINA XII. DNA HYBRIDIZATION OF FIVE ISOLATES

The nonradioactive labelled probe pSM889 was hybridized to Southern blots of genomic DNA of five isolates of Schistosama japonicum from Anhui, Hubei, Guangxi, Sichuan and Yunnan in the mainland of China. The major bands of hybridization of DNA digested by restriction endonucleases EcoRI and BamHI are same among the five isolates, but the minor bands of hybridization of DNA digested by EcoRI show some differences between 4. 4-9. 6 kb and between 2. 3-4. 4 kb among the five isolates. The result shows that the five isolates are of some genetic variation. It provides, at molecular level, evidence of the existence of different strains of 5. japonicum in the mainland of China.