| 人源乳腺癌单链抗体基因的构建 |
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| 引用本文： | 唐树彬,段红,蒲军,李少林,彭志平.人源乳腺癌单链抗体基因的构建[J].重庆医科大学学报,2006,31(6):787-789. |
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| 作者姓名： | 唐树彬 段红 蒲军 李少林 彭志平 |
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| 作者单位： | 重庆医科大学基础医学院核医学教研室,重庆 400016;重庆医科大学基础医学院核医学教研室,重庆 400016;重庆医科大学基础医学院核医学教研室,重庆 400016;重庆医科大学基础医学院核医学教研室,重庆 400016;重庆医科大学基础医学院核医学教研室,重庆 400016 |
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| 摘 要： | 目的：构建人源抗乳腺癌单链抗体（scfv）基因片断。方法：首先利用乳腺癌患者癌旁淋巴组织提取总RNA，然后通过RT—PCR分别扩增VH和VL基因片断，再将经纯化的VH和VL基因片断通过“重叠-延伸-拼接PCR”（SOE—PCR）而形成单链抗体（scfv）基因片断。结果：构建了750bp的人源抗乳腺癌单链抗体（scfv）基因片断。结论：成功地构建了人源抗乳腺癌单链抗体基因片断，为进一步构建人源抗乳腺癌单链抗体库提供了试验基础。
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| 关 键 词： | 乳腺癌 单链抗体 构建 |
| 文章编号： | 0253-3626（2006）06-0787-03 |
| 收稿时间： | 2006-04-19 |
| 修稿时间： | 2006年4月19日 |
Construction of human single-chain antibody gene for breast cancer |
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| TANG Shubin,et al.Construction of human single-chain antibody gene for breast cancer[J].Journal of Chongqing Medical University,2006,31(6):787-789. |
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| Authors: | TANG Shubin |
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| Institution: | Department of Nuclear Medicine, College of Basic Medicine, Chongq ing Medical University |
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| Abstract: | Objiective:To construct human single-chain antibody gene fragment for breast cancer.Methods:Total RNA was isolated from tumor adjacent lymphatic tissue of breast cancer patients.Then heavy and light chain(VH and VL) genes of antibody were amplified separately by RT-PCR,and assembled into ScFv genes by SOE-PCR.Results:The human single-chain antibody gene fragment for breast cancer was constructed and its size was about 750bp.Conclusion:The human single-chain antibody gene fragments were constructed successfully,which provides a way for constructing the human single-chain antibody library of anti-breast cancer. |
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| Keywords: | Breast cancer Scfv Construction |
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