| RP-HPLC测定板蓝根颗粒中靛玉红的含量 |
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| 引用本文： | 吴泽鹏. RP-HPLC测定板蓝根颗粒中靛玉红的含量[J]. 中国民族民间医药杂志, 2010, 19(6): 78-78,80 |
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| 作者姓名： | 吴泽鹏 |
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| 作者单位： | 广东食品药品职业学院; |
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| 摘 要： | 目的:建立板蓝根颗粒中靛玉红的含量测定方法,为板蓝根颗粒的质量标准控制提供理论依据。方法:采用LC-10ATvp高效液相色谱仪,色谱柱:Diamonsil C18柱(4.6mm×250mm,10μm);流动相:甲醇:0.2%醋酸溶液(73:27);流速:1.0ml/min;检测波长:289nm;柱温:25℃;进样量:20μl。结果:靛玉红进样量在5.28～26.40μg范围内与峰面积呈良好线性关系,R=0.9935;平均回收率为100.67%,RSD=1.31%。结论:本实验所建立的方法专属性强,准确度高,重现性好,可作为板蓝根颗粒的质量控制方法。
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| 关 键 词： | RP—HPLC 板蓝根颗粒 靛玉红 |
Study on Content Measurement of Indirubin in Banlangen Granules by RP-HPLC |
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| Abstract: | Objective： To establish a method for extracting of measuring the content of indirubin in banlangen granules by RP-HPLC, in order to provide theoretical basis for the quality standards of banlangen granules. Methods： The content measurement of indirubin in banlangen granules by RP-HPLC, LC-10ATvp high-performance liquid chromatograph, Columns Diamonsil - C18（4.6mm×150mm, 10μ）, mobile phase： methanol： 0.2% acetic acid solution（73：27）; flow rate： 1.0ml/min; detection wavelength： 289nm： column temperature： 25℃; injection volume 20μl. Results： The indirubin within the range of 5.28～26.40μg peak area was linear, R=0.9935; The average recovery was 100.67%, RSD=1.31%. Conclusion： The established method has strong specificity, high accuracy and good reproducibility, it can be used as quality control of banlangen granules. |
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| Keywords: | RP-HPLC Banlangen Granules indirubin |
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