运动训练动员骨髓内皮祖细胞促进缺血下肢血管新生的研究

目的探讨运动训练对骨髓内皮祖细胞(EPCs)的动员及对缺血下肢微血管生成的影响。方法 SD大鼠60只随机平均分为A、B、C三组,A、B组建立大鼠下肢缺血模型,C组仅作皮肤切开缝合。建模1周后A组大鼠跑步训练(30 min/d)。B、C组日常活动,1周后通过细胞培养,观察外周血EPCs数量的变化,4周后检测缺血组织块免疫组化微血管密度(MVD)的表达。结果 A组外周血培养的EPCs平均为(14.18±2.60)个/HPF,显著高于B组为5.59±1.34/HPF,P<0.01;A组MVD:39.94±4.01/HPF,显著高于B组(27.23±3.64)个/HPF,P<0.01;B组的外周血EPCs、组织MVD均明显高于C组(P<0.01),A、B、C 3组之间存在显著性差异(P<0.01)。结论运动训练能动员骨髓EPC,促进缺血肢体血管形成,改善局部血供。

Study on exercise training stimulating mobilization of bone marrow derived endothelial progenitor cells and neovascularization of ischemic lower limbs

Objective To explore the effect of exercise training(ET) on mobilization of bone marrow derived endothelial progenitor cells(EPCs) and neovascularization of ischemic lower limbs.Methods A total of 60 Sprague-Dawley(SD) rats were equally randomized into groups A,B,and C.Lower limb ischemia was established in groups A and B.Simple skin incision and suturing were conducted in group C.Rats in group A were trained to run 30 minutes a day for 4 weeks,while rats in groups B and C maintained only daily activities.One week after exercise training,the changes of the circulating EPCs were observed by mononuclear cells culture.Microvessel density(MVD) expression of ischemic legs was detected at week 4.Results The number of circulating EPCs cultured more significantly increased in group A compared with group B(14.18±2.60/HPF vs 5.59±1.34/HPF,P<0.01).MVD in group A was significantly higher than in group B(39.94±4.01/HPF vs 27.23±3.64/HPF,P<0.01).The circulating EPCs and tissue MVD in group B were significantly higher than in group C(P<0.01).There were significant differences between groups A,B and C(P<0.01).Conclusion Exercise training can promote neovascularization and improve local perfusion in ischemic tissues by mobilizing bone marrow derived EPCs.