染氡大鼠和铀矿工靶细胞p16基因甲基化研究

目的探讨p16基因甲基化与氡子体累积暴露剂量的关系。方法雄性Wistar大鼠20只,随机分成4组,最低剂量组暴露于普通环境中,低、中、高剂量组整体暴露于多功能生态氡室,氡子体累积暴露剂量分别达1、27、54和111工作水平月(WLM)。91名氡职业暴露工人按氡子体累积暴露剂量分为高(>120WLM)、中(60～120WLM)、低(30～59WLM)和最低(2～29WLM)4个剂量组,用聚合酶链反应-甲基化特异性(MSP)检测4组人群痰细胞和大鼠支气管上皮细胞p16基因甲基化。结果当染氡剂量达到111WLM,大鼠支气管上皮细胞出现p16基因甲基化。在铀矿工中,随着氡子体累积暴露剂量增加,痰细胞p16基因甲基化率呈上升趋势(0.00%～20.00%,P<0.01)。结论大鼠支气管上皮细胞和铀矿工痰细胞p16基因甲基化可能与氡子体累积暴露剂量有关。

Study on methylation of p16 gene in target cell of radon-exposed population and in rats

Objective To study the methylation of p16 gene in the target cells of radon-exposed population and in rats.Methods Twenty male Wistar rats were randomly divided into four groups,one group was exposed in common environment,the other three groups were exposed to multi-functional ecology radon cabinet,with cumulative dose of 1 WLM(the lowest dosage),27 WLM(low dosage),54 WLM(median dosage)and 111 WLM(high dosage),respectively.91 radon-exposed workers were divided into 4 groups,high dosage group(>120 WLM(working level month),medium dosage group(between 60 and 120 WLM),low dosage group(between 30 and 59 WLM)and the lowest dosage group(between 2 and 29 WLM)according to the accumulated exposure dosage of the radon daughters.Aberrant promoter methylation of p16 gene in the target cell of the population and rats was detected with methylation specific PCR(MSP).Results Methylation of p16 gene occurred in rats when the accumulated exposure dosage of the radon daughters reached 111 WLM.There was significant upward trend for the p16 gene methylation rate(0.00 %-20.00 %)with the increase of the accumulated exposure dosage of the radon daughters in uranium miners(P<0.01).Conclusion The methylation of p16 gene could be associated with the accumulated exposure dosage of the radon daughters.