| 用PCR法对布鲁氏菌进行筛查及分型鉴定的研究 |
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| 引用本文： | 李坚,李铁锋,王艾琳. 用PCR法对布鲁氏菌进行筛查及分型鉴定的研究[J]. 中国地方病防治杂志, 2009, 0(4): 300-302 |
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| 作者姓名： | 李坚 李铁锋 王艾琳 |
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| 作者单位： | 北华大学检验学院;吉林省地方病第一防治研究所; |
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| 摘 要： | 目的寻找一种快速检测布鲁菌病的方法,并且用这种方法鉴别布鲁氏菌的种和部分型。方法根据布鲁氏菌编码312 kDa布鲁氏菌蛋白的基因（BSCP31）和IS711插入序列及相邻单染色体DNA种的不同,设计引物,建立AMOS-PCR方法,用于诊断布鲁氏菌病,并鉴定种。结果布鲁氏菌蛋白的基因（BSCP31）为引物可以扩增出牛、羊、猪型布鲁氏菌,用IS711插入序列及相邻单染色体设计的引物可以区别不同型别的布鲁氏菌,最低可检测到1 pg的布鲁氏菌DNA。结论AMOS-PCR方法是一种快速、简便、准确的布鲁氏菌病诊断方法,并能鉴定布鲁氏菌的种。
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| 关 键 词： | 布鲁氏菌 布鲁氏菌病 AMOS-PCR |
Study on the screening and species identification of Brucellahe with PCR assay |
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| LI Jian,LI Tie-feng,WANG Ai-lin. Study on the screening and species identification of Brucellahe with PCR assay[J]. Chinese Journal of Control of Endemic Disenaces, 2009, 0(4): 300-302 |
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| Authors: | LI Jian LI Tie-feng WANG Ai-lin |
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| Affiliation: | Examine College of Beihua University;Jilin 132013 |
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| Abstract: | Objective To search a rapid diagnostic method for use in the screening and species identification of Brucella.Methods The primers used in PCR assay were designed according to the sequence of gene encoding the outer membrane protein(31KDa) and the Brucella insertion sequence IS711 and its neighbouring chromatid DNA sequence in order establish the abbreviated Brucella AMOS-PCR assay.Results The results of The primers of 31 KDa specificity detection showed that the expected gene of Brucella was amplified based... |
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| Keywords: | Brucella Brucellosis AMOS-PCR |
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