U937泡沫细胞中细胞间粘附分子—1的表达及欧芹素乙的抑制作用

目的：研究在人类单核细胞系U937泡沫细胞中,细胞间粘附分子－1（ICAM－1）的表达水平,观测欧芹素乙（欧前胡内酯,imperatorin,IMP)对ICAM－1表达的抑制作用。方法：将U937细胞与80mg/L氧化低密度脂蛋白孵育48h,建立U937泡沫细胞模型,在培养基中预加入不同浓度的IMP（0,25,50,100μmol/L）,采用Western blotting检测ICAM－1的蛋白表达;采用Northern blotting检测ICAM－1的mRNA水平。结果：泡沫细胞中ICAM－1的表达显著高于正常U937细胞。ICAM－1的蛋白和mRNA水平分别是正常U937的15和10倍。经IMP50和100μmol/L预处理后,泡沫细胞中ICAM－1的高表达被显著抑制。当IMP浓度达到100μmol/L时,ICAM－1的蛋白水平降低了79％,mRNA水平降低了74％。结论：经氧化低密度脂蛋白孵育后,U937泡沫细胞中ICAM－1呈现高表达,IMP能显著抑制这种表达。

Expression of intercellular adhesion molecule-1 in U937 foam cells and inhibitory effect of imperatorin

AIM: To investigate the expression level of intercellular adhesion molecule-1 (ICAM-1) in a macrophage-derived foam cell model from human U937 cell line and the inhibitory effect of imperatorin (IMP) on the ICAM-1. METHODS: U937 cells were incubated with oxidized low density lipoprotein (ox-LDL) 80 mg/L for 48 h and a macrophage-derived foam cell model was established. The medium was pretreated with different concentrations of IMP (0, 25, 50, 100 micromol/L). ICAM-1 protein expression in cells was measured with Western blotting; ICAM-1 mRNA level in cells was measured by Northern blotting. RESULTS: After incubated with ox-LDL, ICAM-1 expression level increased greatly. The increase in ICAM-1 protein level and mRNA level was estimated to be about 15-fold and 10-fold. When the cells were pretreated with imperatorin (50, 100 micromol/L), the increase of ICAM-1 in foam cells were remarkably inhibited. Especially when pretreated with IMP 100 micromol/L, the ICAM-1 protein level decreased by 79 % and the mRNA level decreased by 74 % each compared to the level of foam cells. CONCLUSION: After incubated with ox-LDL in vitro, the U937 foam cells showed an enhanced ICAM-1 expression compared with normal U937 cells. IMP could inhibit the expression of ICAM-1 in U937 foam cells.