慢性乙型肝炎患者HBV DNA载量与血清IL-2、IL-6、IL-8、IL-13水平的相关性研究

目的探讨慢性乙型肝炎病毒(HBV)感染患者血浆HBV DNA载量与乙肝血清标志物的关系及与白细胞介素2(IL-2)、白细胞介素6(IL-6)、白细胞介素8(IL-8)、白细胞介素13(IL-13)含量变化的相关性。方法选取60例慢性HBV感染者(疾病组),采用免疫发光法定量检测乙型肝炎标志物;实时荧光定量PCR(FQ-PCR)检测HBV DNA载量,并以5.0×102copies/ml为临界点分为高载量组、低载量组;酶联免疫吸附试验(ELISA)检测IL-2、IL-6、IL-8、IL-13含量;并与20名健康对照者(正常对照组)比较。结果疾病组血浆HBV DNA载量5.0×102copies/ml时,以HBs Ag、抗-HBe、抗-HBc 3项指标升高为主要表现,阳性检出率为95%,IL-2、IL-6、IL-8、IL-13水平显著高于正常对照组(P0.05);血浆HBV DNA5.0×102copies/ml时,以HBs Ag、HBe Ag、抗-HBc 3项指标升高为主要表现,疾病组阳性检出率为70%,抗-HBe阳性检出率为30%;疾病组血清IL-2、IL-6、IL-8、IL-13水平显著高于对照组(P0.05),并显著高于血浆HBV DNA低载量组(P0.05)。结论 HBV DNA载量和细胞因子水平的变化可反应机体免疫活动状况,对临床观察疗效具有重要意义。

Study on correlation between HBV DNA load in chronic hepatitis B patients and IL-2, IL-6, IL-8, IL-13 level in serum

Objective To investigate the plasma of patients with chronic hepatitis B virus （HBV） infection of HBV DNA loads and the relationship between hepatitis B serum marker and IL-2,IL-6,IL-8,IL-13 content.Methods Sixty patients with chronic HBV infection （disease group）.Hepatitis B markers were detected by immune luminescence assay HBV DNA loads was detected by real-time quantitative PCR （FQ-PCR）,and ＜ 5.0 × 102copies/ml for the critical point was divided into two groups：high load group and low load group.The IL-2,IL-6,IL-8,IL-13 content were detected by enzyme-linked immunosorbent assay （ELISA）.And compared with 20 cases of healthy controls （control group）.Results HBV DNA ＜ 5.0 × 102 copies/ml in disease group,with HBsAg,anti-HBe,anti-HBc indexes increased as the main performance,the positive rate was 95％,IL-2,IL-6,IL-8,IL-13 levels in serum were significantly higher than those in control group （P ＜0.05）.The plasma HBV DNA ＞ 5.0 × 102copies/ml,with HBsAg,HBeAg,anti-HBc indexes increased as the main performance,the positive detection rate was 70％,anti-HBe positive rate was 30％.The serum levels of IL-2,IL-6,IL 8,IL-13 were significantly higher than those in control group （P ＜ 0.05）,and significantly higher than the low load group （P ＜ 0.05）.Conclusion HBV DNA load and changes in cytokine levels can response the body＇ s immune activity,which is of great significance for the clinical treatment.