| 益寿丸对衰老小鼠脑线粒体DNA缺失突变的影响 |
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| 引用本文： | 吴立蓉,黄张杰,黄兆胜.益寿丸对衰老小鼠脑线粒体DNA缺失突变的影响[J].海峡药学,2014(5):21-23. |
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| 作者姓名： | 吴立蓉 黄张杰 黄兆胜 |
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| 作者单位： | [1]广东药学院药用生物活性物质研究所,广州510006 [2]广东省生物活性药物研究重点实验室,广州510006 [3]广州中医药大学中药学院,广州510006 |
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| 基金项目： | 广东省自然科学基金博士启动项目（S2012040007921）;广东省中医药局建设中医药强省科研课题（20121156） |
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| 摘 要： | 目的：研究益寿丸对D-半乳糖（ D-gal）所致衰老小鼠线粒体损伤的影响，探讨其抗衰老的作用机制。方法采用 PCR法，考察益寿丸对D-半乳糖致衰老模型小鼠脑线粒体DNA缺失突变的影响；并在透射电镜下对衰老动物脑组织线粒体的超微结构进行观察。结果模型组、益寿丸低剂量组及阳性药组小鼠海马mtDNA中存在着约3.87kb片断缺失。益寿丸高、中剂量组未见缺失片断。结论提示益寿丸有防御模型小鼠脑mtDNA缺失突变的作用，益寿丸在调节能量代谢方面具有一定改善作用。
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| 关 键 词： | 益寿丸 衰老 线粒体DNA |
Effect of YiShou Pills on Brain Mitochondrial DNA Missing of the Aging Model Mice |
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| WU Li-rong,HUANG Zhang-jie,HUANG Zhao-sheng.Effect of YiShou Pills on Brain Mitochondrial DNA Missing of the Aging Model Mice[J].Strait Pharmaceutical Journal,2014(5):21-23. |
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| Authors: | WU Li-rong HUANG Zhang-jie HUANG Zhao-sheng |
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| Institution: | 1. Institute of Pharmaceutical Bioactive Substance Research, Guangdong Pharmaceutical University, Guangzhou 510006, China ;2. Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances,Guangzhou 510006 , China ; 3. School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou .510006, China) |
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| Abstract: | OBJECTIVE To study the YiShou Pills on brain mitochondrial DNA missing of the aging model mice ,to explore the mechanism of its anti-aging.METHODS PCR was used to inspect the effect of YiShou Pills on brain mitochondrial DNA missing of the aging model mice induced by D-gal;And ultrastructure of aging mice brain mitochondria were observed under the transmission electron microscope.RESLUTS The results showed that model group,YiShou Pills low dosage group and positive controlled group carried 3.87kb mtDNA deficiency in brain of mice.While YiShou Pills high and middle dosage group could not carried mtDNA deficiency.CONCLUSION YiShou Pills could defense the brain mitochondrial DNA missing of the aging model mice ,which had the improvement action of regulating energy metabolism. |
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| Keywords: | YiShou Pills Aging Mitochondrial DNA |
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