HPLC法测定宫瘤清片中苦杏仁苷、黄芩苷的含量

目的：建立HPLC法测定宫瘤清片中苦杏仁苷、黄芩苷含量的方法。方法采用AgiLent 1100系列高效液相色谱仪，以AgiLent HC-C18色谱柱（4.6mm×250mm，5μm）分离，体积流量1mL· min -1，柱温30℃。①苦杏仁苷：流动相为甲醇-水（20∶80），检测波长210nm；②黄芩苷：流动相为甲醇-0.2％磷酸溶液（47∶53），检测波长280nm。结果苦杏仁苷在18～180μg· mL -1范围内线性关系良好（r＝0.9996），平均回收率为99.61％，RSD＝1.02％（n＝9）；黄芩苷在6.657～66.57μg· mL -1范围内线性关系良好（r＝0.9997），平均回收率为101.49％，RSD＝1.11％（n＝9）。结论本方法操作简便，结果准确，重现性好，可作为宫瘤清片的质量控制方法。

Detetmination of Amygdalin and Baicalin in Gongliuqing Tablets by HPLC

OBJECTIVE To establish a method for Amygdalin and Baicalin in Gongliuqing Tablets by HPLC.METHODS The analysis method was established using AgiLent 1100 HPLC system,the analysis was car-ried out with an AgiLent HC-C18 column（4.6mm ×250mm,5μm）,the flow rate was 1mL· min-1 and the column temperature maintained at 30℃.①Amygdalin：the mobile phase of methanol-water（20∶80）,the detection wavelength was set at 210nm;②Baicalin：the mobile phase of methanol-0.2 phosphoric acid（47∶53）,the detection wavelength was set at 280nm.RESULTS The linear ranges were 18-180μg· mL-1 for Amygdalin（r=0.9996）,the average recoveries were 99.61%and the RSD were 1.02%（ n=9）;the linear ranges were 6.657-66.57μg· mL-1 for Ba-icalin（r=0.9997）,the average recoveries were 101.49%and the RSD were 1.11%（n=9）.CONCLUSION The method is simple ,accurate ,and reproducible.It can be used to control the quality of Gongliuqing Tablets.