被动吸烟对孕鼠及胚胎发育的影响

目的探讨被动吸烟对孕鼠及胚胎发育影响。方法将妊娠Wistar大鼠暴露于被动吸烟,每天4次,每次10min,连续20d,于第1、10、20d称体重。第20d测孕鼠血清NO浓度,观察死胎、吸收胎发生情况,测量胎鼠体重、身长、尾长、肝重、脑重、宫内发育迟缓情况。结果实验组(n=105)死胎和吸收胎、胎鼠宫内发育迅缓发生率分别为24.76%和53.16%,均高于对照组(9.09%,5.55%),孕鼠血清NO浓度显著低于对照组。实验组胎鼠体重、身长、尾长、脑重均低于对照组胎鼠(P<0.01)。结论被动吸烟抑制孕鼠体内NO的生成也抑制了胚胎发育,对胚胎发育有不利影响。

Effects of Passive Smoking on Pregnant Rats and Embryo Development

Objective To explore the effects of passive smoking on pregnant rats and embryo development.Methods The pregnant Wistar rats were exposed to passive smoking4times per day,10min per time,continuously for20days,and the body weights were measured at the1st day,10th day and20th day.At the at20th day,the concentrations of NO in serum of pregnant rats were determined,simultansly the fetal death,embrgonic absorption were observed,the body weights,body length,tail length,brain weight and live weight of fetuses fetal growth retardation(IUGR)were observed.Results Significantly higher frequencies of fetal death,embryonic absorption,IUGR and significantly lower concentration s of NO in serum of pregnant rats were found in experiment group exposed to passive smoking.Significantly lower levels of body weight,body length,tail length,brain weight of fetuses were found in experiment group exposed to passive smoking compared with those of controls(P<0.01)Conclusion Passive smoking inhibited the formation of NO in serum of pregnant rats exposed to passive smoking and embryo develpment,and adverse effects on embryo develpment.