苦参碱隐形脂质体的包封率测定和体外释放度研究

目的:研究苦参碱隐形脂质体的包封率测定方法,考察其体外释放度。方法:采用硫酸铵梯度法制备苦参碱隐形脂质体;用葡聚糖凝胶G-100柱分离脂质体和游离药物,并用反相高效液相色谱法测定脂质体的包封率;用《中国药典》中溶出度第二法测定其体外释放度。结果:制得脂质体的包封率为52.40%;苦参碱检测浓度的线性范围为0.005～0.200mg·mL-1(r=0.999 9);平均回收率为98.36%(RSD=0.95%);脂质体的体外释放规律符合Higuchi方程。结论:本方法简单、快速、准确、重现性好,可用于该脂质体的包封率和体外释放度的测定。

Determination of Entrapment Efficiency of the Stealth Matrine Liposomes and Study on Its Release in Vitro

OBJECTIVE： To establish a determination method of entrapment efficiency for the stealth matrine liposomes and to investigate its release profile in vitro. METHODS： Stealth matrine liposomes were prepared using ammonium sulfate transmembrane gradients method; Free matrine and liposomes were separated by Sephadex gel G- 100 column; RP- HPLC was applied to determine the entrapment efficiency; The release behavior of the drug from liposomes was studied by the second method for dissolution stated in Chinese Pharmacopeia. RESULTS： The entrapment efficiency of the stealth matrine liposomes was 52.40%; The calibration curve showed good linearity for matrine in the range of 0.005--0.200 mg·mL^-1（r = 0.999 9） and the average recovery rate was 98.36% （RSD = 0.95% ）; The release rule in vitro was subject to the Higuchi model. CONCLUSIONS： The established method is rapid, accurate, reproducible, and suitable for the determination of the entrapment efficience and in vitro release.