葡萄籽提取物原花青素诱导乳腺癌 MCF-7 细胞脱落凋亡

目的 检测葡萄籽提取物原花青素诱导乳腺癌MCF-7细胞脱落凋亡的作用。方法 采用DNA ladder检测及软琼脂集落形成试验方法，观察乳腺癌MCF-7细胞对脱落凋亡的敏感性以及原花青素诱导其脱落凋亡的作用。结果 MCF-7细胞具有抗脱落凋亡的特性，而0．1mmol／L原花青素即可引起悬浮培养的MCF-7细胞凋亡。表现为细胞染色质DNA断裂及软琼脂集落形成受阻。结论 原花青素可诱导乳腺癌MCF-7细胞脱落凋亡。

Inducing effect of proanthocyanidin from seed of Vitis vinifera on anoikis of breast cancer cells MCF-7

Object To study the inductive effect of proanthocyanidin from the seed of Vitis vinifera L. on anoikis of breast cancer cells MCF 7. Methods DNA laddering assay and soft agar assay were used to observe the sensitivity of MCF 7 cells to anoikis and the inductive effect of proanthocyanidins on anoikis. Results MCF 7 cells had the peculiarity of anoikis resistance, and proanthocyanidins at concentration of 0.1 mmol/L could remarkably induce apoptosis of suspended MCF 7 cancer cells. The chromatin DNA cleavage could be detected by agarose gel electrophoresis and the cell's potential of forming colonies was suppressed. Conclusion Proanthocyanidins from the seed of V. vinifera can induce anoikis of the breast cancer cell MCF 7.