| 猪苓多糖对膀胱癌细胞癌基因蛋白表达的影响 |
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| 引用本文： | 曾星,章国来,梅玉屏,黄羽,何盛琪.猪苓多糖对膀胱癌细胞癌基因蛋白表达的影响[J].中国肿瘤临床,2003,30(2):81-83. |
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| 作者姓名： | 曾星 章国来 梅玉屏 黄羽 何盛琪 |
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| 作者单位： | 广州中医药大学第二附属医院,广州市,510120 |
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| 基金项目： | 国家自然科学基金(编号:39870944)，高等学校优秀教师基金资助(2001)39 |
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| 摘 要： | 目的：分析猪苓多糖对体外培养T24膀胱癌细胞癌基因蛋白表达的影响。方法：应用免疫荧光法、激光共聚焦显徽镜对p53、H—ras基因蛋白表达进行定位、定量观察．结果：猪苓多糖显著增加p53蛋白表达，24小时表达最高，呈弥漫性分布，随后逐渐下降；对照组在24小时表达很弱，48小时表达最强。猪苓多糖对H—ras基因蛋白表达无明显影响。结论：猪苓多糖对T24细胞p53基因蛋白表达有一定的调节作用，对H-ras基因蛋白表达无明显影响。
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| 关 键 词： | 膀胱癌 猪苓多糖 癌基因 免疫荧光法 激光共聚焦显微镜 p53基因 H－ras基因 蛋白表达 |
| 文章编号： | 1000-8179(2003)02-0081-03 |
| 修稿时间： | 2001年4月2日 |
The Expression of P53 and H-ras in Bladder Cancer Cells Induced by Polyporus Polysaccharide |
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| Zeng Xing Zhang Guolai Mei Yuping et al Second Affiliated Hospital,Guangzhou University of TCM,Guangzhou China.The Expression of P53 and H-ras in Bladder Cancer Cells Induced by Polyporus Polysaccharide[J].Chinese Journal of Clinical Oncology,2003,30(2):81-83. |
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| Authors: | Zeng Xing Zhang Guolai Mei Yuping Second Affiliated Hospital Guangzhou University of TCM Guangzhou China |
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| Abstract: | Objective:To elucidate the specific mechanisms of the expression of P53and H-ras in bladder cancer cells induced by polyporus polysaccharide(PPS).Methods:Laser scan-ning confocal microscope was used to investigate the distribution of the p53?H-ras protein in t24bladder cancer cells.Results:p53labeled with fluorescence spread all over the cytosol and nuclei in the T24cells after24h treatment with PPS,while the fluorescent intensity decreased after48h stimulation;No correlation with expression of H-ras in T24cells was found.Conclu-sion:These results suggested enhanced expression of P53of bladder cancer cells induced by PPS. |
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| Keywords: | Polyporus polysaccharide Bladder cancer |
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