bFGF和celecoxib对胃癌BGC-823细胞株生长的影响及其机制

目的：探讨bFGF和celecoxib对人胃癌BGC-823细胞增殖及凋亡的作用及对COX-2、NF-κB、VEGF蛋白表达的影响.方法：bFGF和celecoxib作用于BGC-823人胃癌细胞后，MTT比色法测定细胞的增殖，使用流式细胞仪检测细胞凋亡率，荧光显微镜观察细胞凋亡形态，应用Western印迹分析法检测COX-2、NF-κB和VEGF蛋白的表达.结果：25ng/ml bFGF对BGC-823细胞有促增殖作用，能增强COX-2、NF-κB、VEGF蛋白表达，呈时间依赖性（P＜0.01）.不同浓度的celecoxib对BGC-823人胃癌细胞均有增殖抑制作用，并能促进细胞凋亡，使COX-2、NF-κB、VEGF蛋白表达明显减弱，呈浓度依赖性（P＜0.01）.结论：bFGF对BGC-823细胞具有促进增殖和COX-2、NF-κB、VEGF蛋白表达的作用.celecoxib对BGC-823细胞具有促进凋亡和抑制COX-2、NF-κB、VEGF蛋白表达的作用.COX-2、NF-κB、VEGF之间具有相关性（r分别为0.852、0.908、0.930，P＜0.01）.

Effects of bFGF and Celecoxib on Growth in BGC-823 Gastric Carcinoma Cells

Objective:To investigate the effects of bFGF and celecoxib on proliferation,apoptosis and expression of COX-2,NF-KB,and VEGF protein in BGC-823 gastric carcinoma cells.Methods: BGC-823 gastric carcinoma cells were treated with bFGF and celecoxib.Proliferation,apoptosis,mor- phology and expression of COX-2,NF-KB,and VEGF protein were observed using MTT,flow cytome- try,fluorescence microscopy and Western blotting,respectively.Results:bFGF promoted BGC-823 cell proliferation and expression of COX-2,NF-KB and VEGF protein at a concentration of 25 ng/ml in a time-dependent manner(P<0.01).Celecoxib at different concentrations inhibited proliferation of BGC- 823 cells and induced apoptosis.The expression of COX-2,NF-KB and VEGF protein was attenuated in a dose-dependent manner(P<0.01).Conclusion:bFGF can promote BGC-823 cell proliferation and expression of COX-2,NF-KB,and VEGF protein.Celecoxib promotes apoptosis of BGC-823 cells and inhibits expression of COX-2,NF-KB and VEGF protein in a dose-dependent manner.COX-2,NF- KB and VEGF show interaction during the progression of carcinoma(r=0.852,0.908 and 0.930,respec- tively;P<0.01).