人胎儿胸腺内CD80和癌胚抗原的表达及定位

目的 探讨人胎儿胸腺内CD80和癌胚抗原(CEA)的表达水平及定位与胎儿发育的关系. 方法自附属医院妇产科收集流产死亡的胎儿12例,其中,小于4个月的4例列为早期,5个月的8例列为晚期.早晚期标本各分为两等份,一份制备冷冻切片进行HE染色、α-醋酸萘酯酶(ANAE)组织化学染色、CD80及CEA免疫组织化学染色,另一份以Trizol提取蛋白后进行CD80的免疫印迹和CEA的酶联免疫吸附测定(ELISA)检测. 结果 早期胎儿胸腺髓质发育差,晚期胎儿胸腺CD80阳性网状上皮细胞可分为6型:Ⅰ型扁平细胞分布于被膜内面和小梁表面;Ⅱ型呈小星形分布于皮质内;Ⅲ型及Ⅳ型呈扁平状,位于皮髓质交界处;Ⅴ型呈大星形状,突起多而细长相连成网状;Ⅵ型位于哈氏小体.在胎儿胸腺髓质网孔处可见ANAE点型(CD+CD8-)和散粒型(CD4-CD8+)细胞各呈丛状分布.早期组CEA阳性分布于髓质上皮细胞和哈氏小体,晚期组CEA阳性更集中于哈氏小体.CD80的免疫印迹和CEA的ELISA检测皆显示晚期组表达高于早期组. 结论 研究结果提示,人胎儿胸腺内CD80和CEA的表达及定位与胎儿胸腺细胞的发育及功能密切相关.

Expression and localization of CD80 and CEA in human fetal thymus

Objective To explore the relationship between the expression level and localization of CD80 and carcinoembryonic antigen(CEA) in the human fetal thymus and its development. Methods Twelve aborted dead fetus samples(via donation from the dead fetus’s parents and permission of the hospital agency) were collected from Department of Genecology and Obstetrics in the affiliated hospital, including 4 cases aged less than 4 months in earlier stage and 8 cases aged 5 months in later stage. The two groups of earlier and later aged samples were randomly divided into two aliquots. One aliquot was prepared for frozen sections stained with HE, ANAE histochemistry, CD80 and carcinoembryonic antigen (CEA) immunohistochemistry; and detected with CEA ELISA. The protein extracted from another aliquot with Trizol reagent was detected with CD80 immunoblotting and CEA ELISA. Results The thymus in earlier aged group, especially the medulla was less developed. The reticulo-epithelial cells (RECs) with CD80 IR in the later aged group could be classified into six patterns. The type I RE in flatten shape were located at the inner surface of the capscule and trabecula. The type II RECs (DCs) in small satellite shaped were localized in the medulla. Both type III and type IV RECs were located at the dzemarcation between the cortex and medulla. The type V RECs (DCs) in large satellite shaped with delicate processes to connect into reticular meshes were distributed in the medulla and the type VI RECs were found in the Hassall’s corpuscles. The ANAE dot pattern T cells (CD4SUP>+ /SUP>) and ANAE-diffuse granular pattern T cells (CD8SUP>+/SUP> ) in separate clusters could be found in the reticular pores. In the immunoblotting and ELISA the expression level of both CD80-IR and CEA-IR in the later aged group was higher than that in the earlier aged group.Conclusion The results suggest that the expression and cocalization of CD80 and CE