青龙衣多糖对H22型肿瘤细胞的ATPase活性影响的研究

目的 研究青龙衣多糖对H22型肿瘤细胞的ATPase活性的影响。方法 按照测定无机磷含量的方法对H22肿瘤细胞的Ca^2＋-ATPase、Na^＋K^＋-ATPase和Mg^2＋-ATPase活性进行了测定。结果 发现青龙衣多糖可以降低ATPase的活性，除青龙衣多糖中、高剂量组显著抑制Ca^2＋-ATPase活性。高、中、低剂量组都显著降低Na^＋-K^＋-ATPase和Mg^2＋-ATPase活性（P〈0．05）。结论 ATPase活性降低导致了膜电位下降。细胞容积降低．可能引起细胞凋亡发生．Ca^2＋．调节凋亡发生．Mg^2＋调控ATPase的活性,可能是青龙衣多糖抗肿瘤的机制之一。

Research on effect of ATPase in tumor cells of H22 type with Qinglongyi Polysaccharide

Objective To study the effect of ATPase in tumor cells of H22 type with Qinglongyi Polysaccharide. Method ATPase test kits are used to determine the activities of Ca2+-ATPase?Na+K+-ATPase and Mg2+-ATPase in tumor cells of H22 type. Results It showed Qinglongyi polysaccharide high-, middle-, low-dosage group could decrease the activity of Na+-K+-ATPase and Mg2+-ATPase besides the high-, middle- dosage group could inhibit the activity of Ca2+-ATPase(P<0.05). Conclusion The decrease of ATPase makes the membrane potential and the cell volume reduce they lead to apoptosis. Ca2+ can regulate apoptosis, and Mg2+ regulate the activity of ATPase. So the Qinglongyi Polysaccharide may kill the tumor by the decrease of ATPase.