| 斑点免疫结合试验诊断侵袭性阿米巴病 |
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| 引用本文： | 郭增柱,纪爱华,卢恩奇,王正仪,吕兴侠,陈明,吴文漪,俞幼知. 斑点免疫结合试验诊断侵袭性阿米巴病[J]. 徐州医学院学报, 1990, 0(1) |
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| 作者姓名： | 郭增柱 纪爱华 卢恩奇 王正仪 吕兴侠 陈明 吴文漪 俞幼知 |
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| 作者单位： | 北京热带医学研究所(郭增柱，纪爱华，卢恩奇，王正仪)，徐州医学院附院传染科(吕兴侠，陈明，吴文漪)，徐州医学院附院传染科(俞幼知) |
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| 摘 要： | 应用斑点免疫结合试验(DIA)检测64例侵袭性阿米巴病患者的血清阿米巴抗体,42例阿米巴肝脓肿和1例阿米巴肺脓肿患者均呈阳性反应,滴度分布为1:16～1:8192;21例肠阿米巴病(痢疾和肠炎)患者的抗体滴度为1:4～1:2048,其中19例阳性。139例对照中有3例呈阳性反应。和ELISA相比较,本试验节约抗原和材料,操作简便,适于一般实验室和流行病学调查现场应用。
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| 关 键 词： | 阿米巴病 免疫诊断 斑点免疫 结合试验 |
DOT IMMUNOBINDING ASSAY ( DIA ) ON NITROCELLULOSE MEMBRANE AS A TOOL FOR DIAGNOSING INVASIVE AMOEBIASIS |
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| Guo Zengzhu. DOT IMMUNOBINDING ASSAY ( DIA ) ON NITROCELLULOSE MEMBRANE AS A TOOL FOR DIAGNOSING INVASIVE AMOEBIASIS[J]. Acta Academiae Medicinae Xuzhou, 1990, 0(1) |
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| Authors: | Guo Zengzhu |
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| Abstract: | Dot immunobinding assay ( DIA ) on nitrocellulose membrane as a useful tool was used for diagnosing Entamoeba histolytica infections. The soluble antigen used in the assay was made from the axenic culture of E.histolytica. Serum samplex from 42 cases of amebic liver abscess, 1 emebic pulmonary abscess, 21 acute intestinal amoebiasis and 139 various controls ( normal, other parasitic infections and other diseases ) were subjected to this assay. All cases of extra-intestinal amoebiasis and 90.5% of intestinal amoebiasis showed positive reaction, and 2.1% of various control samples showed cross reaction. The sensitivity and specificity of this assay are as good as that of ELISA, but DIA is simpler, quicker and cheaper than the latter. Hence, it is a serological test of choice for diagnosing amoebiasis. |
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| Keywords: | amoebiasis immunodiagnosis dot immunobinding |
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