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Characterization and cross-species amplification of 11 polymorphic microsatellite loci in Liobagrus marginatoides |
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| Authors: | Xiang-Yang Jia Ying-Wen Li Deng-Qiang Wang Hui-Wu Tian Bin Tu Da-Qing Chen |
| Affiliation: | 1. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, No. 8, 1st Wudayuan Road, East Lake Hi-Tech Development Zone, Wuhan, 430223, Hubei, People’s Republic of China 2. College of Life Sciences, Chongqing Normal University, Chongqing, 401331, People’s Republic of China 3. Xianning No. 1 People’s Hospital, Xianning, 437100, People’s Republic of China |
| Abstract: | Liobagrus marginatoides is endemic to the upper Yangtze River basin in China. By using the fast isolation by amplified fragment length polymorphism of sequences containing repeats protocol, we identified eleven microsatellite loci in a wild population. The number of alleles per locus ranged from 4 to 10. The observed and expected heterozygosity ranged from 0.3258 to 1.000 and from 0.6506 to 0.8712, respectively. Cross-amplification was tested in related species L. nigricauda, L. marginalus and L. kingi. |
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