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Development of 11 polymorphic microsatellite markers for Xylocarpus granatum (Meliaceae) using next-generation sequencing technology |
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| Authors: | Yuki Tomizawa Yoshimi Shinmura Alison K. S. Wee Koji Takayama Takeshi Asakawa Orlex Baylen Yllano Severino Garengo Salmo III Erwin Riyanto Ardli Nguyen Xuan Tung Norhaslinda Binti Malekal Onrizal Sankararamasubramanian Halasya Meenakshisundaram Sarawood Sungkaew Mohd Nazre Bin Saleh Bayu Adjie Khin Khin Soe Emiko Oguri Noriaki Murakami Yasuyuki Watano Shigeyuki Baba Edward L. Webb Tadashi Kajita |
| Affiliation: | 1. Department of Biology, Graduate School of Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba, 263-8522, Japan 2. Department of Biological Sciences, National University of Singapore, Singapore, Singapore 3. The University Museum, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan 4. Biology Department, College of Sciences and Technology, Adventist University of the Philippines, Silang City, Cavite, Philippines 5. Department of Environmental Science, School of Science and Engineering, Ateneo de Manila University, Quezon City, Philippines 6. Faculty of Biology, Jenderal Soedirman University, Purwokerto, Indonesia 7. Mangrove Ecosystem Research Centre, Hanoi National University of Education, Hanoi, Vietnam 8. Institute for Tropical Biology and Conservation, Universiti Malaysia Sabah, Kota Kinabalu, Malaysia 9. Forestry Sciences Department, Universitas Sumatera Utara, Medan, Indonesia 10. Biotechnology Programme, Swaminathan Research Foundation, Chennai, India 11. Faculty of Forestry, Kasesart University, Bangkok, Thailand 12. Department of Forest Management, Faculty of Forestry, Universiti Putra Malaysia, Selangor, Malaysia 13. Bali Botanical Garden, Bali, Indonesia 14. Department of Botany, University of Yangon, Yangon, Myanmar 15. Department of Biological Sciences, Tokyo Metropolitan University, Tokyo, Japan 16. Tropical Biosphere Research Center, University of the Ryukyus, Iriomote, Okinawa, Japan |
| Abstract: | Human impacts have seriously damaged mangroves, and conservation of mangroves will require information on local and regional population genetic structures. Here, we report the development and polymorphism of eleven novel microsatellite markers, developed using next-generation sequencing on 56 samples of widespread mangrove species Xylocarpus granatum (Meliaceae) from nine populations across the Indo-West Pacific region. All loci were found to be polymorphic, with the number of alleles per locus ranging from four to 19. In a population from Sabah (Malaysia), the mean observed and expected heterozygosity per locus was 0.59 and 0.58, respectively. No null allele, significant linkage disequilibrium or deviation from Hardy–Weinberg equilibrium was detected among all loci. The eleven markers developed can be valuable tools to conservation genetics of this species across its distributional range. |
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