胃癌患者血清淋巴管内皮生长因子检测的临床相关性研究

目的检测胃癌患者血清淋巴管内皮生长因子（VEGF-C）的水平,评估其作为诊断和预测胃癌患者淋巴结转移指标的临床意义。方法采用酶联免疫吸附试验法（ELISA）检测70例胃癌患者与20例正常对照者血清中VEGF-C的水平。结果胃癌患者血清VEGF-C水平为（1586.04±315.13）pg/m L,明显高于正常对照组的（868.33±82.43）pg/mL（P〈0.01）;胃癌淋巴结转移组血清VEGF-C水平为（1802.80±182.45）pg/m L,明显高于无淋巴结转移组的（1187.50±211.42）pg/mL（P〈0.01）;按照有无脉管浸润分组,浸润组血清VEGF-C水平明显高于无浸润组（P〈0.01）;TNM分期为Ⅲ期和Ⅳ期组血清VEGF-C水平明显高于TNM分期为Ⅰ期和Ⅱ期组（P〈0.05）;胃癌患者血清VEGF-C水平与其他临床病理参数无关（P〉0.05）;血清VEGF-C水平高于1423.20 pg/m L预测淋巴结转移的灵敏度和特异度分别达78.95%和72.22%。结论血清VEGF-C水平可作为胃癌的生物学指标,为胃癌患者术前判断淋巴结是否转移和临床分期提供新的诊断依据。

Clinical Correlation of Serum VEGF- C Level in Patients with Gastric Cancer

Objective To detect the serum lymphatic vascular endothelial growth factor（VEGF- C） level,and to evaluate its clinical significance as the index for diagnosing and predicting lymphonodus metastasis in the patients with gastric cancer. Methods The enzymelinked immunosorbent assay（ELISA） was adopted to detect the serum VEGF- C level in 70 cases of gastric cancer and 20 healthy controls. Results The serum VEGF- C level in the gastric cancer group was（1586. 04 ± 315. 13） pg/m L,which was obviously higher than（868. 33 ± 82. 43） pg/m L in the normal control group（P〈0. 01）; the serum VEGF- C level in the lymphonodus metastasis group was（1802. 80 ＋ 182. 45） pg/m L,which was obviously higher than（1 187. 50 ± 211. 42） pg/m L in the non- lymphonodus metastasis group（P〈0. 01）; according to with or without lymphatic vessel invasion,the serum VEGF- C level in the lymphatic vessel invasion group was significantly higher than that in non- lymphatic vessel invasion group（P〈0. 01）; the serum VEGF- C level in the TNM stage Ⅲ,Ⅳ group was significantly higher than that in the TNM stage Ⅰ,Ⅱ group（P〈0. 05）; the serum VEGF- C level in the patients with gastric cancer had no relation with other clinical pathological parameters（P〉0. 05）; the sensitivity and specificity of serum VEGF- C level 〉1 423. 20 pg/m L for predicting lymphonodus metastasis reached to 78. 95% and 72. 22% respectively. Conclusion The serum VEGF- C level can be used as a biological index for gastric cancer,which provides a new diagnostic basis for judging lymphonodus metastasis and clinical staging before operation in the patients with gastric cancer.