两种精浆酸性磷酸酶检测方法的比较与评价

目的:对检测精浆酸性磷酸酶(ACP)活性的常规方法(磷酸苯二钠法)和试剂盒方法进行比较,并探讨试剂盒方法取代常规方法用于常规检测的可能性。方法:79份不育男性的精浆,分别用常规方法和试剂盒方法检测其ACP活性。取1份精浆标本用于两种方法的批内检测,另取2份标本用于两种方法的批间检测。随机留取10份精浆标本,稀释后立即或放置30 m in后检测ACP活性。另随机留取10份精浆标本,同时由2位技术人员分别用常规方法检测ACP活性。结果:试剂盒方法所测ACP活性与常规方法显著相关(r=0.745,P=0.000)。试剂盒方法与常规方法相比,两者批内CV(13.72%;10.66%)比较接近,而批间CV(13.8%和15.49%;24.43%和21.04%)明显较低。精浆稀释后放置30 m in,无论是常规方法还是试剂盒方法,所测结果都显著降低(P<0.05)。而2位技术人员用常规方法所测精浆ACP活性无显著区别(P=0.165)。结论:试剂盒方法检测精浆ACP活性优于常规方法,可以取代常规方法。

Comparison and Evaluation of Two Methods for Measureing Acid Phosphatase Activity in Seminal Plasma

Objective: To compare the routine method and the kit method for the measurement of acid phosphatase activity in seminal plasma,and to explore the possibility of the kit method for routine measurement. Methods: Seventy-nine seminal plasma samples were assayed by routine method and kit method respectively for acid phosphatase.One sample was detected 10 times for within-run analysis,and an other two were measured by both the methods once a day for 10 days for between-run analysis.Acid phosphatase activities in another 10 seminal plasma samples collected at random were measured immediately or 30 min after dilution by two technicians,respectively. Results: There were significant positive correlations between the acid phosphatase activities measured by routine and kit methods(r=(0.745),P=(0.000)).In the within-run assay,the coefficient of variation for the kit method((13.72)%) was similar with that for the routine method((10.66)%).But in the between-run assay,the coefficients of variation for the kit method((13.8)% and(15.49)%) were obviously lower than those for the routine method((24.43)% and(21.04)%).Compared with the acid phosphatase activities in seminal plasma measured immediately after dilution,those measured after 30-min standing were notably lower for either of the methods(P<(0.05)).However,there wasn't significant difference in the acid phosphatase activities detected by the routine method between the two technicians(P=(0.165)). Conclusion: The kit method is superior and preferable to the routine method for the measurement of acid phosphatase in seminal plasma.