Investigation of the adjuvant effect of polyethylene glycol (PEG) 400 in BALB/c mice. |
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| Authors: | S?ren T Larsen Gunnar D Nielsen Peter Thygesen |
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| Affiliation: | 1. National Institute of Occupational Health, Lersø Parkallé 105, DK-2100, Copenhagen, Denmark;2. Department of Pharmacology, Royal Danish School of Pharmacy, Universitetsparken 2, DK-2100, Copenhagen, Denmark;1. Genomics Research Center, Academia Sinica, Nankang, Taipei, 115, Taiwan;2. Institute of Biotechnology, National Taiwan University, Taipei, 106, Taiwan;3. Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, 112, Taiwan;4. Department of Internal Medicine, National Taiwan University Hospital, Taipei, 100, Taiwan;1. Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, 11751 Nasr City, Cairo, Egypt;2. Analytical Chemistry Department, Faculty of Pharmacy, Modern University for Technology and Information (MTI), 12582 Al Hadaba Al Wosta, Cairo, Egypt;1. Protein Research Laboratory (LabInPro), Faculty of Natural Sciences and Surveying (FACENA), National Northeastern University (UNNE), Corrientes 3400, Argentina;2. Department of Biochemistry, Institute of Biology (IB), State University of Campinas (UNICAMP), Campinas, SP, Brazil;3. Laboratory of Pharmacology, Faculty of Veterinary Science, National Northeastern University (UNNE), Argentina;1. Department of Ophthalmology and Visual Sciences, University of Iowa;2. Department of Pediatrics, University of Iowa;3. Department of Biostatistics, University of Iowa |
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| Abstract: | In the formulation of peptide- and protein-based drugs, it is important that the pharmaceutical excipients used do not potentiate possible immunogenic properties of the drug substance. Polyethylene glycols (PEGs) are widely used excipients e.g. in parenteralia and in formulations for nasal application. The immunomodulating properties of PEG 400 were investigated in this study using hen egg ovalbumin (OA) as the model immunogen. OA was dissolved in saline, 10% PEG 400 in saline or undiluted PEG 400 and injected subcutaneously into the neck region of BALB/cJ mice. The levels of OA-specific IgE, IgG1 and IgG2a antibodies were measured. The 10% solution of PEG 400 did not have any immunomodulating properties, whereas the undiluted product gave rise to immunosuppression when compared with the saline control. Neither 10% nor the 100% PEG 400 preparation possessed adjuvant activity under the conditions of the study. |
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