电针"足三里"对大鼠胃电及脑肠肽的影响

目的:通过电针"足三里"对大鼠胃电及脑肠肽影响的观察,进一步探讨足三里与胃运动内在联系的物质基础.方法:对24只健康成年大鼠胃内埋置电极记录胃电,观察电针对胃电的影响,实验结束后抽取血液1.5 Ml和胃窦组织,采用放免分析法测定血浆和胃窦内的P物质、胃动素(MLT)、胃泌素(GAS)含量的变化.结果:电针"足三里"后大鼠胃电慢波高活动相平均振幅变化率和慢波高活动时程/周期比值,以及快波峰簇数差值比均明显高于对照组(P<0.05,P<0.01).胃窦内脑肠肽物质含量均不同程度高于对照组(P<0.05,P<0.01).结论:电针"足三里"可使胃电活动增强,同时胃活动的增强与相关脑肠肽含量增加有同步效应.

Effects of electroacupuncture at Zusanli (ST 36) on electric activities of the stomach and brain-gut peptides in rats

ObjectiveTo explore substance basis of internal relati on s between Zusanli (ST 36) and gastric movement. Methods The electr ogastrogram was recorded in 24 healthy adult rats by implanted electrodes and th en effects of electroacupuncture on the electric activities of the stomach were investigated. After the experiment was completed, 1 5 mL blood and gastric antr um tissue were taken, and contents of substance P, motilin (MLT) and gastrins (GAS) in plasma and the gastric antrum tissue were determined by redioimmuoassay. ResultsThe mean amplitude change rate of the slow wave high active phase, the slow wave high active time course/cycle ratio, and difference of rapid wave peak numbers in electrogastrogram after acupuncture at Zusanli (ST 36) were significantly higher than those in the control group (P<0 05,P<0 01); and the contents of the brain-gut peptides in the gastric antrum all were higher than those of the control group in varying degrees (P<0 05,P<0 01). ConclusionElectroacupuncture at Zusanli (ST 36) can strengthen electric activities of the stomach and the strengthening of the electric activities is synchronous with the increase of the brain-gut peptide contents.