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| 高效液相色谱法测定沙梅消渴片中牛蒡苷的含量 | |
| 引用本文： | 武立忠,王路宏,牛赫. 高效液相色谱法测定沙梅消渴片中牛蒡苷的含量[J]. 中国卫生产业, 2012, 9(15): 6-7 |
| 作者姓名： | 武立忠 王路宏 牛赫 |
| 作者单位： | 1. 吉林油田总医院,吉林松原,138000 2. 吉林省四平市食品药品检验所,吉林四平,136000 3. 吉林省四平市中心医院,吉林四平,136000 |
| 摘 要： | 目的测定沙梅消渴片中牛蒡苷的含量。方法采用高效液相色谱法,色谱柱:AICHROMTMReliaSil C18 5μm 150mm×4.6mm;流动相:乙腈-水(25:75);流速:1.0mL/min;检测波长:280nm;理论板数按牛蒡苷峰计算应不低于3000。结果在0.5576~2.788μg范围内,峰面积积分值与进样量呈良好线性关系,回归方程为Y=880321.3773X+163975.8,相关系数r=0.9997。平均回收率为96.68%(n=6),RSD%=1.35%。结论该法专属性强,灵敏度高,稳定性好,可作为沙梅消渴片制剂质量控制的定量方法。 |
| 关 键 词： | 沙梅消渴片 牛蒡苷 高效液相色谱法 含量测定 |
High performance liquid chromatographic method for the determination of Sha Mei Xiaoke tablet burdock glycoside content |
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| Abstract: | Objective Determination of sand plum Xiaoke tablet burdock glycoside content Methods Using high performance liq- uid chromatography Chromatographic column ：AICHROMTMReliaSil C18 5 m 150 4.6mm; mobile phase： acetonitrile - water （ 25： 75）; flow： 1.0mL/min; Detection of wavelength： 280nm; the number of theoretical plates by arctiin peak calculation should not be less than 3000. Results In 0.5576 G - 2.788 - g range, the peak area integral value and amount Of sample showed good linear re- lationship, the regression equation was Y=880321.3773X＋163975.8, correlation coefficient r = 0.9997. The average recovery rate was 96.68% （ n = 6, RSD% = 1.35% ）. Conclusion The method of strong specificity, high sensitivity, good stability, can be used as sand plum Xiaoke tablet preparation quality control quantitative method. |
| Keywords: | Sha Mei Xiaoke tablet Burdock Glycosides HPLC Content determination |
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