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| 黄体酮对大鼠实验性变应性脑脊髓炎的治疗作用 | |
| 引用本文： | 余洪俊,费军,罗雪,姚忠祥. 黄体酮对大鼠实验性变应性脑脊髓炎的治疗作用[J]. 第三军医大学学报, 2009, 31(22): 2232-2236 |
| 作者姓名： | 余洪俊 费军 罗雪 姚忠祥 |
| 作者单位： | 1. 第三军医大学西南医院康复科,重庆,400038;第三军医大学基础医学部组织学与胚胎学教研室,重庆市神经科学研究所,重庆,400038 2. 大坪医院野战外科研究所创伤中心,重庆,400042 3. 第三军医大学基础医学部组织学与胚胎学教研室,重庆市神经科学研究所,重庆,400038 |
| 基金项目： | 国家自然科学基金面上项目，重庆市自然科学基金 |
| 摘 要： | 目的 研究黄体酮对实验性变应性脑脊髓炎(experimental allergic encephalomyelitis,EAE)大鼠的治疗作用.方法 采用注射豚鼠脊髓匀浆的方法 制作雄性Wistar大鼠EAE模型.当EAE大鼠出现神经症状后,开始在治疗组腹腔内注射溶于2.5 ml芝麻油中的黄体酮(4 mg·kg~(-1)·d~(-1),连续注射4 d),模型组注射等量的芝麻油.分别对两组大鼠进行运动功能评分和脑组织的电镜检查、LFB染色、锇酸Marchi's染色.染色结果 采用IPP图像分析软件计算光密度(OD)值.结果 治疗组和模型组神经功能评分无明显区别,但是在开始治疗后第6、10、25天,治疗组正常髓鞘的积分光密度值明显高于模型组,而退变髓鞘的积分光密度值明显低于模型组.结论 黄体酮有利于EAE大鼠中枢神经系统的髓鞘再生,促进髓鞘修复. |
| 关 键 词： | 实验性变应性腩脊髓炎 黄体酮 髓鞘再生 |
Therapeutic effect of progesterone for rats with experimental allergic encephalomyelitis |
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| YU Hong-jun,FEI Jun,LUO Xue,YAO Zhong-xiang. Therapeutic effect of progesterone for rats with experimental allergic encephalomyelitis[J]. Acta Academiae Medicinae Militaris Tertiae, 2009, 31(22): 2232-2236 | |
| Authors: | YU Hong-jun FEI Jun LUO Xue YAO Zhong-xiang |
| Affiliation: | YU Hong-jun1,2,FEI Jun3,LUO Xue2,YAO Zhong-xiang2 (1Department of Histology and Embryology,College of Basic Medical Sciences,2Department of Rehabilitation,Southwest Hospital,Third Military Medical University,Chongqing 400038,3Trauma center,Institute of Surgery Research,Daping Hospital,Chongqing 400042,China) |
| Abstract: | Objective To study the therapeutic effect of progesterone for rats with experimental allergic encephalomyelitis(EAE).Methods The male Wistar rats were immunized with guinea pig's spinal cord homogenate.After neurological symptoms occurred in the rats,intraperitoneal injection of progesterone which was dissolved in 2.5 ml sesame oil was given to the therapeutic group at the dose of 4 mg/kg once a day for 4 d and the control group was given the same amount of sesame oil.All the rats were graded with the nerve... |
| Keywords: | experimental allergic encephalomyelitis progesterone remyelination |
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