肾上腺髓质素在肺癌患者中的表达及作用

目的:探讨肾上腺髓质素(adrenomedullin,ADM)在肺癌患者的表达及其与肺癌临床生物学特征的相关性。方法:采用免疫组织化学和放免法检测肺癌组织及血浆中ADM的表达情况,并回顾性分析ADM基因表达与肺癌的临床生物学特征的关系。结果:肺癌组织的ADM蛋白表达明显高于癌旁组织及正常肺组织(P<0.01),而癌旁组织的ADM阳性表达率与正常肺组织差异无统计学意义(P>0.05)。肺癌组织中ADM蛋白的表达与肺癌分化程度有一定关系(P<0.05),与年龄、病理类型和TNM分期无明显关系(P>0.05)。肺癌组血浆中ADM含量均高于非肺癌组和健康对照组(P<0.05和P<0.01),且血浆中ADM的表达与TNM分期有一定关系(P<0.05),与病理类型无明显关系(P>0.05)。结论:肺癌患者中ADM表达明显增强,其表达水平与肺癌分化程度显著相关,ADM基因在肺癌的发生、发展中可能起重要作用。

Expression and role of adrenomedullin in human lung cancer

Objective：To investigate the expression of adrenomedullin （ADM） in human lung cancer and to evaluate its clinical significance. Methods： Immunohistochemical staining was performed to examine the expression of ADM in lung cancer, radioimmunoassay was applied to determine the concentration of ADM in the plasm of patients and controls, and the relationship was retrospectively analyzed between the expression of ADM in lung cancer and the clinicobiological features. Results：The expression of ADM in lung cancer was significantly higher than those in adjacent tissues and normal tissues （P 〈 0.01 ）, and the positive rate of ADM protein in adjacent tissues and that in the normal ones was no significant difference（ P 〉 0.05 ） . There was a significant relationship between the expression of ADM and the grade of lung cancer（ P 〈 0.05 and P 〈 0.01 ）, and there were no relationships between the expression of ADM and age, histological type and TNM stage （ P 〉 0.05 ）. The expression of ADM in the plasm of lung cancer group was significantly higher than those in the plasm of non-lung cancer group and healthy control group （ P 〈 0.05 ）. There was a significant relationship between the expression of ADM in the plasm and TNM stage （ P 〈 0.05 ）, and there was no relationships between the expression of ADM in the plasm and histological type （P 〉 0.05 ）. Conclusions. The expression of ADM significantly increased in lung cancer and was associated with grade. ADM may play an important role in the lung cancer.