基质金属蛋白酶-3血清水平及启动子基因5A/6A多态性与动脉粥样硬化性脑梗死的关系

目的 探讨摹质金属蛋白酶-3(matrix metalloproteinase-3,MMP-3)血清水平及启动子基因5A/6A多态性与动脉粥样硬化性脑梗死(atherosclerotic cerebral infarction,ACI)的关系.方法 对215例ACI患者和226名健康体检者进行研究.采用ELISA法测定血清MMP-3水平,同时采用聚合酶链反应-限制性片段长度多态性方法分析MMP-3启动子基因5A/6A多态性.结果 ACI组发病48 h内的血清NIVIP-3水平为(23.28±12.19)ng/μL,较对照组(2.76±2.25)ng/μL显著增高(t=24.867,P=0.000).Aa组内5A/6A+5_4/5.4基因型患者外周血MNP-3水平为(26.04±11.79)ng/μg,6A/6A基因型患者外周血MMP-3水平为(22.24±12.21)ng/mL,两者间差异有统计学意义(t=2.057,P=0.041).ACI组5A./6A+5A/5A基因型频率为27.4%,对照组为15.5%,两者比较差异有统计学意义(x2=9.389,P=0.1102),5A等位基因频率在ACI组为14.2%,对照组为7.7%,两者比较差异也有统计学意义(X2=9.430,P=0.002).结论 ACI患者发病48h内血清MMP-3水平显著增高,MMP-3启动子基因5A/6A多态性可能与MMP3的血清水平及ACI发生倾向性有关.

Association of matrix metalioproteinase-3 serum level and the promoter 5A/6A polymorphism of the MMP-3 gene with atherosderotic cerebral infarction

Objective To investigate the association of matrix metalluproteinase-3 (MMP-3) serum level and the promoter 5A/6A polymorphism of theMMP-3 gene with atherosclerotic cerebral infarction (ACI) in a Chinese Hart population.Methods Two hundred and fifteen patients with acute ACI from the Department of Neurology of Taizhou Hospi tal and 226 healthy controls were included in the study.Serum MMP-3 level was measured by enzyme-linked immunosorbent assay (ELISA).Genotype was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for the common 5A/6A functional promoter polymorphism of the MMP-3 gene.Results The genotype distribution of the MMP-3 promoter 5A/6A polymorphism between the ACI patients group and the control group was significantly different(X2=9.389,P=0.002).The frequencies of the 5A allele were 14.2%and 7.7%in the ACI patients group and the control group respectively(X2=9.430,P=0.002).Serum level of MMP-3 in the ACI patients group was significantly higher than that in the control group(t=24.867,P=0.000).Among the ACI patients group,serum MMP3 levels also had significant difference between the 5A/6A + 5A/SA and the 6A/6A genotype(t=2.057,P=0.041).Conclusion The present findings suggest that serum level of MMP-3 obviously increased within 48 hours of ischemic stroke and the genetic polymorphism of 5A/6A in the MMP-3 promoter is associated with ACI and MMP-3 expression in the Chinese Han population.