复方积雪草2号对IgA肾病大鼠肾组织电镜形态及Nephrin表达的影响

目的：观察复方积雪草2号对IgA肾病（IgAN）大鼠肾组织病理电镜及Nephrin表达的影响。方法：复合免疫法制造IgAN大鼠模型,分设正常对照、模型、模型＋缬沙坦、模型＋复方积雪草2号高剂量、模型＋复方积雪草2号中剂量、模型＋复方积雪草2号低剂量6组。观察各组大鼠肾脏病理及肾组织Nephrin表达的变化。结果：（1）造模各组大鼠尿蛋白、镜下红细胞增多;免疫荧光镜下造模各组肾小球系膜区有不同程度IgA荧光,提示造模成功;（2）电镜下模型组系膜细胞增生,基质明显增多、有足突融合;而各治疗组足突融合情况均较模型组明显改善（P〈0.05）;（3）免疫组化法检测Nephrin显示模型组几乎没有阳性表达;RT-PCR显示模型组NephrinmRNA表达明显弱于正常组和其他各治疗组（P〈0.01）。结论：（1）Nephrin的下降可能是IgAN足细胞损伤、产生蛋白尿的病理机制之一;（2）Nephrin可能是复方积雪草2号、缬沙坦发挥肾小球足细胞保护作用的分子靶点之一。

Effect of Fufangjixuecaoerhao Ⅱ on Renal Electron Microscope Pathology and Nephrin Expression by Podocyte of Rats with IgA Nephropathy

Objective.To observe the effect of fufangjixuecaoerhao on renal pathology with electron microscope and expression of nephrin by podocyte of rats with IgA nephropathy. Methods：The IgAN rat model was induced by multiple immune. 6 groups were divided： control, model, valsartan, and three dosages （ high, middle and low） of fufaugjixuecaoerhao. Changes of renal pathology and immuno - fluorescent were observed by microscope. The expression of nephrin was detected by immunohistochemistry and RT - PCR. Results： （1） Albuminuria and hematuria were observed in all five model groups. Fluoroscope showed IgA density in mesangial area in five model groups. These suggested that the model establishing was succeeded; （2） Mesangioproliferative, electron dense deposition and podocyte fusion were observed in model group by electromicroscopy, while those in all treatment groups were not remarkable; （3） It showed yellowish- brown positive nephrin expression in all treatment groups. The nephrin mRNA expression was obviously slight in model group than that in control group and all treatment groups by RT-PCR （P〈0.01）. Conclusion： （1） Decrease of nephrin expression is involved in pathogenesis of podocyte injury and proteinuria in IgAN; （2） nephrin might be the molecular target of poodocyte protection by fufaugjixuecaoerhao and valsartan.