| 人釉原蛋白重组质粒PcDNA3-AMG在COS-1细胞系中的表达 |
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| 引用本文： | 章锦才,赵川江,徐琛蓉. 人釉原蛋白重组质粒PcDNA3-AMG在COS-1细胞系中的表达[J]. 口腔医学, 2006, 26(6): 401-403 |
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| 作者姓名： | 章锦才 赵川江 徐琛蓉 |
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| 作者单位： | 广东省口腔医院·南方医科大学附属口腔医院牙周科,广州,510280;中山大学光华口腔医学院牙周科,广州,510055 |
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| 基金项目： | 广东省自然科学基金重点资助项目(36946) |
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| 摘 要： | 目的研究人釉原蛋白(AMG)重组质粒PcDNA3-AMG在COS-1细胞系中的表达。方法采用脂质体载体法将釉原蛋白重组质粒PcDNA3-AMG导入COS-1细胞系,用Zeoin筛选得到稳定转染克隆,并经ELISA检测细胞内和细胞培养液中重组釉原蛋白AMG的表达。结果在未经转染的对照组细胞内和细胞培养液中均未检测到AMG的表达,而经转染的实验组不论细胞内或细胞培养液中均检测到AMG的较高表达,重组质粒PcDNA3-AMG转染组细胞内AMG浓度达0.253μg/ml。结论PcDNA3-AMG具有较强的在真核细胞系中表达和分泌AMG的能力,适宜基因工程体外制备重组釉原蛋白。
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| 关 键 词： | 釉原蛋白 COS-1细胞 转染 真核表达 |
| 文章编号： | 1003-9872(2006)05-0401-03 |
| 收稿时间： | 2006-05-15 |
| 修稿时间： | 2006-05-15 |
Study on expression of recombinant plasmid PcDNA3-AMG for human amelogenin in COS-1 cell line |
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| ZHANG Jin-cai,ZHAO Chuan-jiang,XU Chen-rong. Study on expression of recombinant plasmid PcDNA3-AMG for human amelogenin in COS-1 cell line[J]. Stomatology, 2006, 26(6): 401-403 |
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| Authors: | ZHANG Jin-cai ZHAO Chuan-jiang XU Chen-rong |
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| Abstract: | Objective To investigate the amelogenin expression of recombinant plasmid PcDNA3-AMG in COS-1 cell line.Methods PcDNA3-AMG was used to transfect COS-1 cells firstly.An appropriate concentration of Zeoin was chosen for the selection of stably transfected cell colonies.The ELISA technique was applied to detect the expression of recombinant amelogenin.Results The amelogenin was detected in the stably transfected COS-1 cells in both cell culture supernatant and cell homogenate,and the concentration of expressed amelogenin in PcDNA3-AMG transfected cell homogenate was as high as 0.253 μg/ml.Conclusions The results provided basis for the preparation of recombinant AMG by PcDNA3-AMG transfected eukaryrotic cell line COS-1. |
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| Keywords: | amelogenin COS-1 transfection eukaryotic expression |
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