| 解脲支原体第一群的分型研究 |
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| 引用本文： | 孔繁荣,周向昭,马燕燕.解脲支原体第一群的分型研究[J].中华皮肤科杂志,1999,32(3):155-157. |
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| 作者姓名： | 孔繁荣 周向昭 马燕燕 |
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| 作者单位： | 北京医科大学第一医院皮肤科(孔繁荣!100034北京，马燕燕!100034北京，朱学骏!100034北京)，张家口医学院附属第一医院皮肤科(周向昭) |
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| 摘 要： | 目的 利用分子生物学方法对解脲支原体第一群进行分型，建立简便，可靠的分型方法。方法 设计分型引物，用ＣＲ和限制性内切酶消化的方法进行分型。结果 设计了解脲支原体的分型引物ＵＭＳ５１１ＵＭＡＵ１和ＵＭＳ５１１ＵＭＡＵ３，前者扩增出Ｕｕｌ型和Ｕｕ６型，后者扩增出Ｕｕ３型和Ｕｕ１４型。
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| 关 键 词： | 解脲支原体 血清分型 分子生物学 |
Serotyping of Biovar One of Ureaplasma Urealyticum by Molecular Biological Methods |
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| KONG Fanrong,ZHOU Xiangzhao,Ma Yanyan,et al.Serotyping of Biovar One of Ureaplasma Urealyticum by Molecular Biological Methods[J].Chinese Journal of Dermatology,1999,32(3):155-157. |
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| Authors: | KONG Fanrong ZHOU Xiangzhao Ma Yanyan |
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| Institution: | KONG Fanrong,ZHOU Xiangzhao,Ma Yanyan,et al Department of Dermatology,First Hospital,Beijing Medical University,Beijing 100034 |
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| Abstract: | Objective To set up serotyping methods of Ureaplasma urealyticum by molecular biological techniques Methods Primers for typing were designed and different serotypes of Ureaplasma urealyticum were identified by PCR and restriction endonuclease digestion Results PCR primers UMS51, UMAU1 and UMS51,UMAU3 was specific to Uu1,Uu6 and Uu3, Uu14 respecitively To distinguish Uu1 and Uu6, restriction endonuclease digestion were used Conclusion By PCR and restriction endonuclease digestion on the basis of DNA sequence of N terminal of multiple banded antigen gene, serovars of biovar one of Uu can be distinguished but Uu3 and Uu14 can not be distinguished |
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| Keywords: | Ureaplasma urealyticum Serotyping |
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