参附芎泽方对心肌梗死后心力衰竭模型大鼠心肌能量代谢的影响

目的研究参附芎泽方对心肌梗死(心梗)后心力衰竭(心衰)模型大鼠的心肌能量代谢的影响。方法 SD大鼠分为假手术组、模型组、参附芎泽方药组、曲美他嗪组。采用结扎大鼠冠状动脉前降支建立心梗后心力衰竭模型,假手术组和模型组给予纯净水,参附芎泽方药组和曲美他嗪组分别给予参附芎泽方(含生药量为8.4 g/kg)和曲美他嗪(含药量6.3 g/kg)相应的等体积药液。以20 m L/kg的容积灌胃,1次/d,连续28 d。在末次给药后1 h,腹主动脉采血,离心,取血清,采用ELISA法检测血清CK、CK-MB、FFA含量,Western blot检测心肌组织AMPK、GLUT4蛋白的表达,HE染色观察心肌组织病理学改变。结果与假手术组比较,模型组大鼠血清CK、CK-MB含量降低,FFA含量升高,心肌组织AMPK、GLUT4蛋白表达均下降(P0.05)。与模型组比较,参附芎泽方组和曲美他嗪组大鼠血清CK、CK-MB含量升高,FFA含量降低,心肌组织AMPK、GLUT4蛋白表达均增高(P0.05)。与曲美他嗪组比较,参附芎泽方组大鼠血清CK-MB含量升高,FFA含量降低,心肌组织AMPK蛋白表达下降(P0.05)。HE染色结果 ,心肌梗死后心力衰竭大鼠心肌组织发生明显形态学改变,心肌灶状坏死,坏死灶内肌纤维溶解,心肌纤维肥大,局部可见断裂、萎缩变性的肌纤维,心肌间质水肿,并见大量炎细胞浸润。曲美他嗪和参附芎泽方给药组可不同程度减轻心肌组织形态学改变。结论参附芎泽方可增加血清CK、CK-MB含量,降低血清FFA含量,通过调控心肌组织AMPK、GLUT4蛋白的表达,减轻心肌组织病理损伤,改善心衰。

Effects of Shenfu Xiongze decoction on myocardial energy metabolism in heart failure rat after myocardial infarction

Objective To study the effects of Shenfu Xiongze decoction on the myocardial energy metabolism in heart failure rats after myocardial infarction.Methods SD rats were divided into sham operation group, model group,Shenfu Xiongze decoction group and trimetazidine group. Adopted to left anterior descending coronary artery ligation is establish the model of heart failure after myocardial infarction. The sham operation group and model group were given pure water,Shenfu Xiongze decoction group and trimetazidine group were given Shenfu Xiongze decoction (containing crude drug 8.4g/kg) and trimetazidine (containing 6.3g/kg) corresponding to the volume of liquid. Fill the stomach with 20 mL/kg, 1 times a day for 28 days. At 1 hour after the last administration, blood was collected from abdominal aorta, blood was centrifuged, and serum was collected. The level of serum CK,CK-MB,FFA was detected by ELISA method. The expression of AMPK,GLUT4 in myocardial tissue was detected by Western blot. The pathological changes of myocardium were observed by HE staining.Results Compared with the sham operation group, the levels of serum CK, CK-MB and the myocardial tissue expression of AMPK and GLUT4 in the model group decreased, the content of FFA increased (P<0.05). Compared with the model group, the Shenfu Xiongze decoction group and trimetazidine group, the serum CK, CK-MB content elevated, FFA content decreased, the expression of AMPK and GLUT4 protein in myocardial tissue were increased. (P<0.05). Compared with trimetazidine group, the Shenfu Xiongze decoction group, the serum CK-MB content was increased, the content of FFA in serum and the expression of AMPK protein in myocardial tissue were decreased (P<0.05). HE staining results: the morphological of myocardial tissue in heart failure rats after myocardial infarction have obvious changes, myocardial necrosis, focal necrosis of muscle fiber in the dissolution, myocardial fiber hypertrophy, muscle fibers with local rupture, atrophy and degeneration, myocardial interstitial edema and inflammatory cell infiltration. trimetazidine and Shenfu Xiongze decoction group can significantly relieve the morphological of myocardial tissue changes. Conclusion Shenfu Xiongze decoction can increase serum CK, CK-MB content, reduce the content of serum FFA, By regulating the expression of AMPK and GLUT4 proteins in myocardium, reduce myocardial tissue injury, improve heart failure.