基因芯片研究针刺人迎穴对SHR主动脉弓基因表达的影响

目的应用基因芯片观察针刺人迎穴对SHR主动脉弓基因表达的影响。方法将20只SHR大鼠随机分为模型组、人迎组,10只WKY大鼠作为空白组。人迎组大鼠双侧人迎穴进行针刺干预,并记录各组大鼠收缩压变化。4周后剪取大鼠主动脉弓,进行基因芯片检测。结果与模型组比较,人迎组大鼠收缩压显著降低(P0.05)。模型组与空白组相比,452个基因表达上调,245个基因表达下调;人迎组和模型组相比,261个基因表达上调,986个基因表达下调。KEGG通路分析显示在模型组VS空白组、人迎组VS模型组分别发现了12条、10条显著表达(P0.05)的差异通路。结论针刺人迎穴降压机制可能与调控高血压血管重构的细胞外基质受体相互作用通路及通路中的相关基因表达有关。

Effect of acupuncture Renying ( ST9 ) on the expression of aortic arch gene in SHR rats by gene chip

Objective This study use aortic arch's gene chip to investigate the internal acupuncture mechanism on SHRs. Methods 20 SHR rats were randomly divided into model group and Renying ( ST9 ) group, 10 WKY rats were taken as blank control group. Renying group rats received acupuncture at ST9. After 4 weeks, all rats were executed and gene chip was used for the determination of gene expression. Results Compared with model group, Renying (ST9) group's systolic pressure were remarkably decreased (P < 0.05). Compared with blank control group, gene array analysis revealed that 452 genes were up-regulated and 245 genes were down-regulated in the model group;Compared with model group, 261 genes were up-regulated and 986 genes were down-regulated in Renying (ST9) group. 12 different KEGG pathway were founded in comparison between model group and blank control group,and 10 different KEGG pathway were founded between Renying group (ST9) and model group (P<0.05). Conclusion Acupuncture at Renying (ST9) could effectively reduce hypertension rats with systolic pressure, pathways related to vascular remodeling in hypertension and its mechanism might be related to the different expression of ECM receptor interaction pathway and some genes in the pathway.