参景固本方抗衰老及调节血脂作用研究

目的研究参景固本方对D-半乳糖致亚急性衰老模型大鼠的影响。方法将60只大鼠随机分为6组,即正常对照组、模型组、参景固本方低、中、高剂量组及金匮肾气丸组,每组10只,全部大鼠(除正常对照组外)按125 mg/kg剂量颈背部皮下注射D-半乳糖建立衰老模型。以参景固本方与金匮肾气丸对比治疗,测定增重率、胸腺指数及脾脏指数,测定脑组织中MDA、T-SOD含量以及血脂变化。结果参景固本方组胸腺指数及脾脏指数均明显升高,与模型组比较,差异具有统计学意义(P0.05,P0.01);与模型组比较,参景固本方中剂量组SOD活性明显升高(P0.05),参景固本方高剂量组MDA含量降低,SOD活性明显升高(P0.01);与模型组比较,参景固本方中剂量组HDL-C含量显著降低(P0.01),参景固本方高剂量组T-CHO含量明显升高(P0.01),HDL-C含量显著降低(P0.01)。结论参景固本方具有一定的抗氧化、抗衰老作用,同时也具有一定的改善血脂平衡的作用。

Effect of Shenjing Guben decoction on aging and regulating blood lipid

Objective To study the effect of Shenjing Guben decoction on D-galactose induced subacute aging rats.Methods 60 rats were randomly divided into the normal control group, the model group, the low, middle, high dose group of Shenjing Guben decoction and Jingui Shenqi pill group. There were 6 groups in total, 10 rats in each group. All rats were injected D-galactose (at the dose of 125 mg/kg) at their subcutaneous tissue of nape, except the rats in normal control group. Comparing the treatment of Shenjing Guben decoction with the treatment of Jingui Shenqi pill, we need to measure the rate of liveweight growth, the index of thymus and spleen and to determine the content of MDA and T-SOD in brain tissue and the changes of blood lipid content.Results The index of thymus and spleen of Shenjing Guben decoction was significantly increased, compared with the model group, the difference is statistically significant group(P<0.05,P<0.01); Compared with the modelgroup, the activity of SOD of Shenjing Guben decoction middle dose group was significantly increased (P<0.05), the content of MDA of Shenjing Guben decoction high dose group was significantly reduced and the activity of SOD was significantly increased (P<0.01); Compared with the model group, the content of HDL-C of Shenjing Guben decoction medium dose group was significantly reduced. (P<0.01), the content of T-CHO of Shenjing Guben decoction high dose group was significantly increased (P<0.01) and the content of HDL-C was significantly reduced P<0.01). Conclusion Shenjing Guben decoction have antioxidation and senile-resistant function, and have a certain effect on improving the balance of blood lipid.