丹参治疗重症急性胰腺炎的血液流变学及其相关指标的实验研究

目的 :观察丹参对犬重症急性胰腺炎 (SAP)的血液流变学及其相关指标的影响。方法 :实验分三组 :正常对照组 (NC)、重症急性胰腺炎组 (SAP)和复方丹参干预组 (TS)。用 5 %牛磺胆酸钠和牛胰蛋白酶混合液胰管注入 ,制备犬SAP模型。TS组于模型制备后 15min和 12h分别静脉滴注复方丹参(2ml/kg)。术后动态观察各组以下指标 :血液流变学、血小板活化因子(PAF)、血栓素A2 (TXA2 )、前列腺素I2 (PGI2 )、内皮素 (ET)和一氧化氮(NO)。结果 :SAP组TXA2 、PGI2 、ET、NO、PAF较NC组显著升高 (P <0 .0 1) ,血液流变学指标也有显著的改变 (P <0 .0 1) ;复方丹参对各项指标均有不同的影响 (P <0 .0 5 )。结论 :血液流变性、炎性介质PAF、二对血管活性物质TXA2 /PGI2 和ET/NO均是介导SAP胰腺血循环障碍的重要因素 ,复方丹参是有效的治疗措施之一。

Experimental Research of Tanshin on Hemorrheological and Related Indexes in Severe Acute Panceatitis

Objective: To evaluate the effects of Tanshin on hemorrheological and related indexes in severe acute panceatitis.Method: Seventeen dogs were divided randomly into three groups:normal control group(NC),severe acute pancreatitis group(SAP) and Tanshin group(TS).SAP models were established by injecting 5% sodium taurocholate and trypsin into the main pancreatic duct.The dogs of TS group were dripped Tanshin (2 ml/kg) intravenously 15 minutins and 12 hours respectively after severe acute pancreatitis induction.The changes of indexes that are hemorrheologic parameters,platelet activating factor(PAF),thromboxane A 2(TXA 2),prostaglandin I 2(PGI 2),endothelin(ET) and nitric oxide(NO) were dynamically determined after operations.Results: During SAP,the level of PAF,TXA 2,PGI 2,ET and NO was progressivel increased( P<0.01 ),and the hemorrheologic parameters were significantly elevated( P<0.01 ).After treated with Tanshin,all the above mentioned changes were prevented in varying degrees( P<0.05 ).Conclusion: The changes of hemorrheologic parameters,PAF,TXA 2,PGI 2,ET and NO level might play key roles in the mechanism of hemocirculatory disorder in dogs with severe acute pancreatitis(SAP).TS might be one of important therapeudtic measures.