Neuropsin regulates an early phase of schaffer-collateral long-term potentiation in the murine hippocampus |
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Authors: | Komai S Matsuyama T Matsumoto K Kato K Kobayashi M Imamura K Yoshida S Ugawa S Shiosaka S |
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Affiliation: | Division of Structural Cell Biology, NAIST, 8916-5 Takayama, Ikoma, Nara, 630-0101 Japan. |
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Abstract: | We found that neuropsin, an extracellular matrix serine protease, has a regulatory effect on Schaffer-collateral long-term potentiation (LTP) in the mouse hippocampus. Bath application of 1-170 nM recombinant neuropsin modulated early phase LTP in the Schaffer-collateral pathway with a 'bell-shape' dose-response curve. The maximum enhancing activity (134% of control LTP) was found at approximately 2.5 nM. Bath application of a neutralizing antibody against neuropsin in the hippocampal slice resulted in a marked inhibition of the tetanus-induced early phase of LTP. The in vivo continuous intraventricular infusion of an antisense oligonucleotide against neuropsin significantly reduced the amplitude of the tetanus-induced early phase of LTP in vitro. Neuropsin did not directly change the N-methyl D-aspartate (NMDA) current. Thus, neuropsin appears to act as a regulatory molecule in the early phase of LTP via its proteolytic function on extracellular matrix rather than affecting NMDA receptor-mediated calcium increase. |
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Keywords: | CA1 ECM LTP mouse neuropsin serine protease tPA |
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