胰腺良恶性疾病组织中Smad4mRNA,PTENmRNA,P73mRNA表达及意义

目的　研究胰腺癌、癌旁上皮和慢性胰腺炎组织中Smad4mRNA ,PTENmRNA ,P73mRNA表达特征及临床病理意义。方法　石蜡包块切片组织原位杂交染色法。结果　 2 0例慢性胰腺炎导管上皮和腺泡上皮Smad4mRNA ,PTENmRNA均为阳性表达 ,但P73mRNA均为阴性表达 ;2 5例胰癌旁上皮仅 1例重度不典型增生者呈Smad4mRNA ,PTENmRNA阴性表达和P73mRNA阳性表达 (均为同一病例 ) ;5 3例胰腺癌Smad4mRNA ,PTENmRNA ,P73mRNA阳性病例分别为 34例 (6 4 % )、31例 (5 8% )和 2 7例 (5 1% ) ;胰腺癌Smad4mRNA ,PTENmRNA阳性率明显低于慢性胰腺炎 (χ2 =9 6 ,P <0 0 0 5 ;χ2 =11 8,P <0 0 0 5 )和癌旁上皮 (χ2 =9 0 ,P <0 0 0 5 ;χ2 =11 4 ,P <0 0 0 5 ) ;而P73mRNA阳性率明显高于慢性胰腺炎 (χ2 =15 2 ,P <0 0 0 5 )和癌旁上皮 (χ2 =16 2 ,P <0 0 0 5 )。高分化腺癌和未转移病例Smad4mRNA ,PTENmRNA阳性率明显高于低分化腺癌 (χ2 =4 4 ,P <0 0 5 ;χ2 =4 7,P <0 0 5 )和转移癌 (χ2 =4 2 ,P <0 0 5 ;χ2 =3 9,P <0 0 5 ) ;而P73mRNA阳性率明显低于低分化腺癌 (χ2 =6 4 ,P <0 0 2 5 )和转移癌 (χ2 =4 7,P <0 0 5 )。Smad4mRNA与PTEmRNA在胰腺癌中表达呈正相关 (χ2 =5 6 ,P <0 0 2 5 ) ,P73mRNA与Sma

Expression of Smad4, PTEN and P73 mRNA in benign and malignant pancreatic lesions and its significance

Objective To investigate the expression of Smad4, PTEN and P73 mRNA in chronic pancreatitis and pancreatic carcinoma and explore its clinicopathological significance. Methods In situ hybridization was used determine the expression in routinely paraffin embedded sections. Results The positive expression of Smad4 and PTEN mRNA and negative expression of P73 mRNA were found in 20 cases of chronic pancreatitis. Among 25 samples of pericarcinous tissues, only 1 had positive expression of Smad4 and PTEN mRNA and negative expression of P73 mRNA. The positive expression rats of Smad4, PTEN and P73 mRNA in 53 cases of pancreatic carcinoma were 64%, 58% and 51%, respectively. The positive expression rates of Smad4 and PTEN mRNA were significantly lower but that of P73 mRNA higher in pancreatic carcinoma than in chronic pancreatitis or pericarcinous tissues (P<0 005). However, the positive expression rates of Smad4 and PTEN mRNA were markedly higher but that of P73 mRNA lower in well differentiated tumor without metastasis than in poorly differentiated tumor with metastasis. In pancreatic carcinoma, positive correlation was found between the expression of Smad4 mRNA and that of PTEN mRNA. Meanwhile, negative correlation of expression was found among Smad4, PTEN and P73 mRNA. Conclusions The expression of Smad4, PTEN and P73 mRNA might have coeffects on the carcinogenesis, progression, biological behaviors and prognosis of pancreatic carcinoma. Therefore, they might be important biological markers for pancreatic carcinoma.