胃腺癌组织中分泌血管活性肠肽的肿瘤细胞研究

目的　研究胃腺癌中是否存在分泌血管活性肠肽 (VIP)的肿瘤细胞 ,探讨VIP在胃腺癌发生发展中的作用。方法　 2 0 0 1- 12～ 2 0 0 2 - 0 5通过免疫组化的方法 ,检测华中科技大学同济医院附属协和医院 15例正常胃窦黏膜、2 0例胃腺癌组织中VIP蛋白的表达情况 ,观察样本中VIP阳性反应的细胞和神经纤维。结果　 8例 (8/2 0 )胃腺癌组织中发现VIP阳性细胞 ,其中高分化 2例 (2 / 5 )、中分化 2例 (2 / 7) ,低分化 4例 (4/ 8)。阳性细胞的检出率与胃腺癌的恶性程度无相关性 (P >0. 0 5 )。阳性细胞形态不规则 ,多数细胞核膜及核着色 ,少数胞浆着色 ,核大而不规则。 6例局限分布于肿瘤组织中 ,2例低分化胃腺癌阳性细胞散在分布于整个肿瘤组织中。胃腺癌组织中未见阳性神经纤维 ,正常胃黏膜可见阳性神经纤维 ,但未发现阳性细胞。结论　 4 0 %胃腺癌组织中含有分泌VIP的肿瘤细胞 ,VIP可能对胃腺癌的发生有重要的影响。

The tumor cells of secreting VIP protein in gastric adenocarcinoma

Objective The aim is to study the expression of vasoactive in testinal peptide (VIP) protein in gastric adenocarcinoma, and to try to evaluate the role of VIP on tumor genesis and progress of gastric adenocarcinoma. Method Using im munohistochemical method, we detected the VIP protein in antrum mucosa of 15 hea lthy controls and gastric tissues of 20 patients with gastric adenocarcinoma, an d o bserved the positive cells and nerve fibers stained by anti-VIP antibody. Result s 8(8/20) tissues of patients with gastric adenocarcinoma had the posit ive cell s stained by anti-VIP antibody. 2(2/5) were from well-differentiated adenocarcin oma tissues, 2(2/7) from moderately and 4(4/8) from poorly differentiated adenoc arcinoma tissues. The positive cell rate was not correlated with t he differentiation degree of adenocarcinoma (p>0.05). The VIP positive cells ha d a large irregular nucleus and irregular cell shape, and most showed nucleus and nucl ear membrane immunoreactivity to anti-VIP antibody. 6 (6/20) showed focal VIP po sitivity, and 2 (2/20) tissues from poorly differentiated gastric adenocarcinoma showed diffuse VIP positivity. The positive cells were wot found in nomal mucos a , but there were positive nerve fibers in normal mucosa. Conclusion 40% gastric adenocarcinoma has secreting VIP tumor cells. This study indicated that VIP mig ht play an important role in tumor genesis of gastric adenocarcinoma.