| HPLC法测定参花消渴茶中葛根素的含量 |
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| 引用本文： | 李虹,曹悦. HPLC法测定参花消渴茶中葛根素的含量[J]. 中国药品标准, 2011, 12(5): 360-362 |
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| 作者姓名： | 李虹 曹悦 |
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| 作者单位： | 辽宁省食品药品检验所,沈阳,110023 |
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| 摘 要： | 目的:建立参花消渴茶中葛根素的含量控制标准。方法:采用高效液相色谱法对处方中的粉葛进行含量测定,色谱柱用Discovery C18(4.6 mm×250 mm),流动相为甲醇-0.1%磷酸溶液(22∶78),检测波长250 nm。结果:葛根素在0.039 8～2.488 0μg范围内呈良好的线性关系(r=0.999 9)。平均回收率为98.6%,RSD为1.1%(n=6)。结论:该方法简便、准确。
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| 关 键 词： | 高效液相色谱法 参花消渴茶 葛根素 含量 |
Content Determination of Puerarin in Shenhua Xiaoke Teas by HPLC |
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| Li Hong and Cao Yue. Content Determination of Puerarin in Shenhua Xiaoke Teas by HPLC[J]. , 2011, 12(5): 360-362 |
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| Authors: | Li Hong and Cao Yue |
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| Affiliation: | ( Liaoning Provincial Institute for Control of Food and Drug, Shenyang 110023) |
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| Abstract: | Objective： To establish an HPLC method for determination of Puerarin in Shenhua Xiaoke Teas. Methods： With Discovery C18 column, the mobile phase was Methanol-0. 1% Phosphoric acid （22： 78）. The detection wavelength was 250 nm. Results： Puerarin sample showed a good relationship in the range of 0. 039 8 - 2. 488 0 μg, r = 0. 999 9. The average recovery of add sample was 98.6% （n =6） with RSD 1.1%. Conclusion： The method is simple and accurate. |
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| Keywords: | HPLC Shenhua Xiaoke Teas Puerarin Content determination |
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