HPLC-MS/MS法测定人血浆中穿心莲内酯的浓度

目的:建立HPLC-MS/MS法测定人血浆中穿心莲内酯的浓度。方法:血浆样品经甲醇沉淀后,以甲醇-水（69:31,v/v）为流动相,流速为0.7 ml·min^-1,色谱柱为Hedera-C₁₈（4.6 mm×150 mm,5μm）,柱温为25℃,选择性离子检测（SIM）,穿心莲内酯[M-H₂O-H]-和内标脱水穿心莲内酯[M-H]-均为m/z 331.10。结果:血浆内源性杂质不干扰待测物测定,穿心莲内酯的线性范围为0.987～148.050 ng·ml^-1,定量下限为0.987 ng·ml^-1,批内、批间精密度（RSD）均<8%。样品稳定性良好。结论:该法灵敏、快速、简便,可用于穿心莲内酯的药动学研究。

Determination of Andrographolide in Human Plasma by HPLC-MS/MS

Objective： To develop an HPLC-MS/MS method for the determination of andrographolide in human plasma. Method： Plasma samples were treated by methanol. Separation was carried out on the column of Hedera-C18 （4. 6 mm × 150 mm,5 μm） at 25 ℃. The mobile phase consisted of methanol-water （69：31 ,v/v） pumped at a flow rate of 0. 7 ml ·min^-1. The target ions were m/z 331.10 for andrographolide and dehydroandrographolide. Result： The intrinsic impurities of plasma did not interfere with the determination. The linear range of andrographolide was 0. 987-148. 050 ng· ml^-1. Lower limit of quantification was 0. 987 ng· ml^-1. The inter-day precision was less than 8%. The stability of plasma samples was promising. Conclusion： The established method is fast, sensitive and accurate, and can be applied in the pharmacokinetic study.