| 小鼠血清补体替代途径溶血活性测定的新方法 |
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| 引用本文： | 孙黔云,叶巧玲,闫银萍.小鼠血清补体替代途径溶血活性测定的新方法[J].中国药理学通报,2011,27(11):1619-1622. |
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| 作者姓名： | 孙黔云 叶巧玲 闫银萍 |
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| 作者单位： | 贵州省中国科学院天然产物化学重点实验室,贵州,贵阳,550002 |
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| 基金项目： | 国家自然科学基金，国家重点基础研究发展计划(973计划)资助项目，贵州省优秀青年科技人才资助项目 |
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| 摘 要： | 目的针对现有补体溶血活性测定方法不适宜测定小鼠血清补体的缺点,构建一种血清用量少、灵敏度高的小鼠血清补体替代途径溶血活性测定的新方法。方法选用具有代表性的3个小鼠品系KM、BALB/c和C57BL/6小鼠的血清补体作为测定材料,以兔红细胞为溶血靶细胞,利用眼镜蛇毒因子(CVF)特异激活补体替代途径的特点,构建和优化小鼠血清补体替代途径溶血活性测定的方法,并采用该方法测定两种抗补体蛋白对小鼠体内血清补体活性的影响。结果以CVF作为激活剂成功构建了测定小鼠血清补体替代途径溶血活性的微量新方法。3个品系小鼠的血清在5～20μl的范围内即可分别达到合适的溶血度。该方法明显减少了血清用量,并能有效提高溶血度。结论基于CVF特异激活补体替代途径的特点而构建的小鼠血清补体替代途径溶血活性测定新方法,血清用量少、灵敏度高、稳定性好,适用于测定小鼠血清补体的溶血活性。
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| 关 键 词： | 小鼠血清 补体溶血活性 补体替代途径 补体激活 眼镜蛇毒因子 抗补体蛋白 |
A new method for measurement of the alternative pathway activity of mouse serum complement |
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| SUN Qian-yun,YE Qiao-ling,YAN Yin-ping.A new method for measurement of the alternative pathway activity of mouse serum complement[J].Chinese Pharmacological Bulletin,2011,27(11):1619-1622. |
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| Authors: | SUN Qian-yun YE Qiao-ling YAN Yin-ping |
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| Institution: | SUN Qian-yun,YE Qiao-ling,YAN Yin-ping(The Key Laboratory of Chemistry for Natural Products,Guizhou Province and Chinese Academy of Sciences,Guiyang 550002,China) |
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| Abstract: | Aim To constitute a highly sensitive method to measure the alternative pathway activity of mouse serum complement by using small amount of serum.Methods Three mouse strains KM,BALB/c,and C57BL/6 were chosen to be the source of mouse serum complement.Based on the fact that the alternative complement pathway could be specifically activated by cobra venom factor(CVF),a simple assay was devised for determining the alternative pathway of mouse serum by using rabbit erythrocytes as target cells.The developed assa... |
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| Keywords: | mouse serum complement hemolytic activity complement alternative pathway complement activation cobra venom factor anticomplementary protein |
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