GC法测定深海鱼油软胶囊中EPA和DHA的含量

目的:采用气相色谱法测定深海鱼油软胶囊中二十碳五稀酸(EPA)和二十二碳六稀酸(DHA)的含量。方法:以正己烷为溶剂制备对照品和供试品溶液,用气相色谱法测定,FID检测器,色谱柱为PEG-20M毛细管柱(30 m×0.32 mm,0.5μm),进样口温度250℃,检测器温度280℃,柱温220℃,载气为N2,直接进样,采用外标法计算。结果:EPA的回归方程为y1=6.999 4×10-4x(r1=0.999 1),线性范围9.9～99μg.mL-1;DHA的回归方程为y2=6.358 7×10-4x(r2=0.999 3),线性范围6.125～61.25μg.mL-1;平均加样回收率EPA为103.4%、RSD=6.46%(n=5),DHA为101.5%、RSD=2.08%(n=5)。结论:该法简便快捷,专属性好,可作为深海鱼油类产品中EPA和DHA含量测定的方法。

Content Determination of EPA and DHA in Deep Sea Fish Oil Soft Capsules by GC

Objective： GC is used to determine the content of Eicosapentaenoic Acid （EPA） and Docosahexaenoic Acid （DHA） in Deep Sea Fish Oil Soft Capsules. Methods： N-hexane is used as the solvent to prepare the reference solution and the sample solution.The analytical column was PEG-20M（30 m ×0. 32 mm, 0. 5 μ m）. The injector temperature was 250 ℃, the detector temperature was 280℃ and the column temperature was 220 ℃. The carrier gas was N2. The calculation method was external standard method and the injection method was direct injection. Results： The standard curve for EPA was y1 =6. 999 4×10^-4xin the range of 9. 9 -99 μg·mL-1 （r1 = 0. 9991 ） and that for DHA was y2 =6. 358 7×10^-4x in the range of 6. 125 -61.25μg·mL-1 （r2 =0. 999 3）. The average recovery of EPA was 103.4% with RSD 6.46% （ n = 5）, and that of DHA wasl01.5% with RSD 2.08% （n = 5）. Conclusion ： The method is accurate, simple and quick, and can be used to determine the content of EPA and DHA in Deep Sea Fish Oil Soft Capsules.